Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 44 results for: CIT

Showing: 1 - 20 of 44 results

  1. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1 - Mammalian...- Mammalian Expression mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C)... Expression pCS2+8CmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus...Mammalian Expression mCitrine-C1 - Mammalian Expression mCitrine-pBAD - Bacterial Expression YPet 517 530 80 ....g. off to on) Excitation and Emission wavelengths after activation. Protein Excitation (nm) Emission ... Fusions (Organized by Color) For each table: Excitation and Emission maximum are listed in nm. Brightness...Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

  2. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...References for each serotype can be found below under Citations. AAV2(Y444F) The AAV2(Y444F) serotype is demonstrated...Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F) When using the AAV2(Y444F...publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency transduction.... 2009 Mar;17(3):463-71. PMID: 19066593 Other citations include: Bogner, et al. 2015. Capsid Mutated Adeno-Associated...publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2011. Novel properties of tyrosine-mutant...Ther . Feb;19(2):293-301. PMID: 21045809 Other citations include: Chen, et al. 2014. Reprogramming adipose... Dev . Sep;26(3):165-76. PMID: 26390090 Other citations include: Scalabrino, et al. 2015. Intravitreal...

  3. CRISPR Guide

    Type
    Collection
    ... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most...targeting multiple genomic locations or enhancing specificity, efficiency, or flexibility of the Cas enzyme...Vectors Engineering the Cas9 Endonuclease CRISPR specificity is determined by both the gRNA sequence and the... design, engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the...many modified versions engineered to increase specificity and nuclease activity available. Engineered Cas9s...cleavage of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands...fidelity of Cas9 Researchers have also enhanced specificity by engineering Cas9 into high fidelity Cas9s ...

  4. Chemogenetics AAV Preps

    Type
    Collection
    ...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Roth...-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Roth ...-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Roth ...pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine none 2, 8 Roth 50476 ...pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine none 5 Roth 50478 pAAV-GFAP-hM3D...element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent...

  5. Antibody Guide

    Type
    Collection
    ... of 10 Antigen Specificity Antibodies are available in two types of antigen specificity: monoclonal, meaning...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a specific... Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling Visualization...variety in the antibody allowing for their extreme specificity occurs in the B cell itself prior to antibody..., and can be used to increase flexibility or specificity in antibody-based methods. Antibodies can recognize...isotype-specific or even isotype subclass-specific. This specificity can be used to ensure secondary antibodies bind...cost-effective. They are useful when epitope specificity is not a concern or in cases where you may want...

  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Amara , Susan Ingram Citrate Nanosensors for in vivo measurements of citrate (CitA) Engineering genetically...inverse fluorescent biosensor for citrate (Citron/Citroff), mitochondrial or cytosolic High-Performance Intensiometric...measurements of citrate concentrations. PLoS One. 2011;6(12):e28245. Uwe Sauer Citrate Direct or inverse...Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically Encoded Fluorescent...Inverse-Response Genetically Encoded Biosensors for Citrate. ACS Cent Sci. 2020 Aug 26;6(8):1441-1450. Robert... reveals a role for pH regulation in hair cell toxicity and function. Dis Model Mech. 2014 Jul;7(7):847...Thomas Knopfel Voltage Detection of single action/excitatory potentials in individual neurons and dendrites...

  7. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...many of which detail work to improve the tool’s specificity, orthogonality, and multiplexibility in various...endogenous loci with unprecedented speed and specificity. Figure 2: An overview of CRISPR and NHEJ/HDR...Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol . ...Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature . 523(7561):481-5. PMID: 26098369 Komor...Rationally engineered Cas9 nucleases with improved specificity. Science . 351(6268):84-8. PMID: 26628643 Wang...

  8. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...generally contained at lower biosafety levels and elicit relatively low immunological effects in vivo . ...non-dividing cells with a low immune response and low toxicity. Although recombinant AAV does not integrate into...AAV is currently limited by its small packaging capacity (∼4.5 kb including ITRs), though there is a great...interest and effort directed toward expanding this capacity. Traditionally, AAV requires the presence of another...

  9. TALEN Guide

    Type
    Collection
    ... to recognize their targets with fairly high specificity. Fifteen years of research has shown that when...zinc fingers within an array can reduce their specificity. Two years ago, groups led by Jens Boch at the...though NN can also bind adenenine with lower specificity). With the template for studying this type of...likelihood of context having a negative effect on specificity is on par with ZF arrays, if not slightly lower...

  10. Viral Vectors

    Type
    Collection
    ...gene delivery in-vivo because of their mild immunogencity. These viruses can direct long-term transgene...genome. AAVs are limited by their small packaging capacity. Adenovirus Plasmids | Guide Adenoviruses are ...preferred for in-vivo studies because of its low immunogenicity. Different types of viruses also vary in the...linked above. Virus Expression Genome Packaging Capacity Virus Size (nm) Cells Infected Target Cell Genome...

  11. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TTCTCAAAATGAGAGGGGtgaatctgaagttcaaCAAACCCAGCAGCACCAA CIT1 TAL3226 & TAL3227 TGAAGTTTAAATATGGAGgtcatggcagtgttaaAGATCTGTCCGCAGTCGA cited2 TAL3228 &...TGGTAGACCGCATGATGGcaatgaaccatggtcgTTTCCCAGATGCTGTGAA cited4a TAL3046 & TAL3047 TGAACCATGGTTCTGCTGgcggaggcctgcatggTTATCGCATGGGAATGAA cited4b TAL3048...

  12. Plan Your Experiment

    Type
    Collection
    ...High-fidelity Cas enzymes increase specificity. Dual-nickase approach increases specificity but is less efficient....PAM sequence will have the highest specificity. To increase specificity, you can also consider using a high-fidelity...

  13. Deisseroth INTRSECT Collection

    Type
    Collection
    ...developed, including additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium...Flp AND NOT Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations...neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons...Andrási T, Deisseroth K, Holmes A, Hájos N. 2019. Excitation of diverse classes of cholecystokinin interneurons...

  14. Immunology Research Plasmids and Resources

    Type
    Collection
    ...MGC126648 CALCB calcitonin-related polypeptide beta CALC2, CGRP-II, CGRP2, FLJ30166 CALCR calcitonin receptor...MLT1 NFAT5 nuclear factor of activated T-cells 5, tonicity-responsive KIAA0827, NF-AT5, NFATL1, NFATZ, OREBP...component 5a receptor 1 C5A, C5AR, C5R1, CD88 CALCA calcitonin-related polypeptide alpha CALC1, CGRP, CGRP-I...receptor CRT, CTR, CTR1 CALCRL calcitonin receptor-like CGRPR, CRLR CAMP cathelicidin antimicrobial peptide ...somatostatin receptor 5 - ST2 suppression of tumorigenicity 2 - STC1 stanniocalcin 1 STC STC2 stanniocalcin...interleukin 9 receptor CD129 ST2 suppression of tumorigenicity 2 - TXLNA taxilin alpha DKFZp451J0118, IL14...polypeptide-related sequence B PERB11.2 NCR1 natural cytotoxicity triggering receptor 1 CD335, FLJ99094, LY94,...

  15. Plasmids for Stem Cell Research

    Type
    Collection
    ...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...every other cell type in the body and have the capacity to give rise to an entire organism. iPSC technology...which lower the risk of mutation and minimize tumorigenicity, an inherent disadvantage of iPS cell technology...Neurons Lentiviral Human Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor ...

  16. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... Plasmids for In Vivo Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604...28.98 pEF.myc.ER-E2-Crimson Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643...5.3 pPAS-E and pK-GAFm Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690...

  17. AAVED

    Type
    Collection
    ...PM Lunch 1:00 PM Promoter/Enhancer Specificity, Serotype specificity, AAV dosing, and Other Forms of Regulation...how to best help contribute to this publication. Citation information for openly shared communication: Adeno-Associated...

  18. Neurodegeneration Research Collection

    Type
    Collection
    ...behavior and emotion, uncontrolled movements, memory deficits, as well as difficulty speaking and swallowing...cause of dementia. Symptoms include memory loss, deficits in decision-making and language abilities. The...Noteworthy: Study aberrant axon initial segment (AIS) plasticity with a motor neuron-specific reporter and a PAX7...

  19. Caltech Systemic Capsids

    Type
    Collection
    ...Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in...publications, please acknowledge Viviana Gradinaru and cite as follows: PHP.eB, PHP.S: Chan et al., 2017 Pubmed...L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For...

  20. Zhang Lab CRISPR Page

    Type
    Collection
    ...non-coding RNA elements capable of programming the specificity of the CRISPR-mediated nucleic acid cleavage....10.1126/science.1231143. PubMed . DNA targeting specificity of RNA-guided Cas9 nucleases. Hsu PD, Scott DA...RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann...
Showing: 1 - 20 of 44 results