We narrowed to 18,509 results for: kars;

1. pRS315e_pGal-dCas9-SH3

   Plasmid

   #79613

   Purpose

   Expresses dCas9-SH3 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   SH3 domain

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BC-A1-013

   Plasmid

   #78701

   Purpose

   A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS EFF-1::V5

   Plasmid

   #133584

   Purpose

   Expresses EFF-1 A full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Avinoam et al Science. 2011 Apr 29;332(6029):589-92. d

   Insert

   EFF-1::V5
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Psicheck2 TNC full length 3'UTR

   Plasmid

   #26995

   Depositor

   Joan Massague

   Article

   Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

   Insert

   TNC Full length 3' UTR (TNC Human)
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pILGFP9E3

   Plasmid

   #185842

   Purpose

   Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BC-A1-005

   Plasmid

   #78692

   Purpose

   A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 721-2µ-staHIS3

   Plasmid

   #40611

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   HIS3 (HIS3 Budding Yeast)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLY134

   Plasmid

   #130962

   Purpose

   Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Anderson promoter J23100

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLLX8

   Plasmid

   #79838

   Purpose

   Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

   Depositor

   Stephen Lory, Cammie Lesser

   Article

   Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

   Type

   Empty backbone

   Use

   Contains yeast counter selection gene, cyh2 and b…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BC-A1-014

    Plasmid

    #78702

    Purpose

    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA-eCA

    Plasmid

    #133010

    Purpose

    pFA carrying a E. coli canA gene (Uniprot: P61517)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    Can (can E. coli)
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHSP70B-CP6-hph

    Plasmid

    #165879

    Purpose

    Basic Hygromycin resistance cassette in Alpha1, lacks physical marker or attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Basic Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA-sfGFP

    Plasmid

    #162689

    Purpose

    pFA vector control expressing sfGFP

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    sfGFP
    

    Use

    Tags

    C terminal 6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    PL tet promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMP-SMYD2_2

    Plasmid

    #36350

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SMYD2 (SMYD2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

15. psecBFP2

    Plasmid

    #62230

    Purpose

    encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.

    Depositor

    Erik Snapp

    Article

    Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;

    Insert

    secBFP2
    

    Use

    Tags

    secbfp2

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. peT3a-AqAdk_MVGDH

    Plasmid

    #18092

    Depositor

    Dorothee Kern

    Article

    Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

    Insert

    adenylate kinase (kad A. aeolicus)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Tyr 52 changed to Cys, Val 145 changed to Cys, …

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pFE-dabAB2

    Plasmid

    #133002

    Purpose

    pFE carrying both the dabA2 (Uniprot: D0KWS7) and dabB2 genes (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA6-rbN1 S77A

    Plasmid

    #32945

    Depositor

    Edward Weinman

    Article

    Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

    Insert

    SLc9a3r1 (SLC9A3R1 rabbit)
    

    Use

    Tags

    T7 tag, his, and partial FLAG

    Expression

    Mammalian

    Mutation

    Change Serine77 to Alanine

    Promoter

    CMV

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPWCh

    Plasmid

    #124238

    Purpose

    Gateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.

    Depositor

    Peter Vilmos

    Article

    Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UASp

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Psicheck2 Sox4 mut 3'UTR oligo

    Plasmid

    #26988

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    Sox4 mut UTR oligo (SOX4 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Mutation

    bp 449-509 of SOX4 3'UTR; mut @ bp 483

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only