We narrowed to 14,002 results for: Kars

1. pLAP-MIS12-KARD

   Plasmid

   #114057

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   MIS12-KARD (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-HaloAKAR2.1-CLC

   Plasmid

   #216492

   Purpose

   Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pits

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.1-CLC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-ER-ExRai-CKAR2

   Plasmid

   #236099

   Purpose

   Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to ER surface.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   CYP450-ExRai-CKAR2
   

   Use

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Nuc-deltaCKAR

   Plasmid

   #222430

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Nucleus delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 Mito(OM)-deltaCKAR

   Plasmid

   #222431

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Mitochondrial outer membrane delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Golgi-deltaCKAR

   Plasmid

   #222428

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Golgi delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-FluoSTEP-AKAR (C/Y)

   Plasmid

   #181862

   Purpose

   FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AKAR (C-Y)
   

   Use

   Tags

   CFP(1-10) and cpVenus(E127)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1C-Fluc-oskar_C

   Plasmid

   #146022

   Purpose

   Insect Expression of oskar-3UTR

   Depositor

   Elisa Izaurralde

   Insert

   oskar-3UTR (osk Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-lambdaN-HA-DmOskar_C

   Plasmid

   #146008

   Purpose

   Insect Expression of DmOskar

   Depositor

   Elisa Izaurralde

   Insert

   DmOskar (osk Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHH0103_KARS_tRNA-anti-codon

    Plasmid

    #109865

    Purpose

    Protein expression and purification of KARS_tRNA-anti-codon

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    KARS_tRNA-anti-codon (KARS1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLAP-MIS12-KARD-3D

    Plasmid

    #114058

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    MIS12-KARD-3D (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    BUBR1-S670D, S676D and T680D

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLAP-MIS12-KARD-3A

    Plasmid

    #114059

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    MIS12-KARD-3A (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    BUBR1-S670A, S676A and T680A

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PRKAR1A gRNA (BRDN0001144984)

    Plasmid

    #77721

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1A (PRKAR1A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PRKAR1A gRNA (BRDN0001146809)

    Plasmid

    #77722

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1A (PRKAR1A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PRKAR1B gRNA (BRDN0001146606)

    Plasmid

    #76838

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PRKAR1B gRNA (BRDN0001144765)

    Plasmid

    #76839

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PRKAR1B gRNA (BRDN0001147089)

    Plasmid

    #76840

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. cytoplasmic EKAR (Cerulean-Venus)

    Plasmid

    #18679

    Depositor

    Karel Svoboda

    Article

    Harvey et al PNAS 2008 Dec 9;105(49):19264-9

    Insert

    ERK Activity Reporter
    

    Use

    Tags

    monomeric Cerulean and monomeric Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

    Plasmid

    #216490

    Purpose

    Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-FLEX-FLIM-AKAR

    Plasmid

    #60445

    Purpose

    Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKAR
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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