Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,063 results for: golden gate assembly

Clear keywords
Showing: 361 - 380 of 1063 results

  4. (P8)TT

    Plasmid
    #251396
    Purpose
    rrnB T1 terminator derived from pTrc99A
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    rrnB T1 terminator derived from pTrc99A
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251396-plasmid-map-sequence-id-503709

    Enlarge

  5. (P5)efe

    Plasmid
    #251384
    Purpose
    Gene encoding Ethylene Forming Enzyme (Pseudomonas syringae)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    efe
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251384-plasmid-map-sequence-id-503704

    Enlarge

  6. (P6)TT

    Plasmid
    #251394
    Purpose
    rrnB T1 terminator derived from pTrc99A
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    rrnB T1 terminator derived from pTrc99A
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251394-plasmid-map-sequence-id-503675

    Enlarge

  7. pAIP4_4HS5

    Plasmid
    #234223
    Purpose
    Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4HS5
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234223-plasmid-map-sequence-id-464188

    Enlarge

  11. pAV10.F3

    Plasmid
    #63199
    Purpose
    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63199-plasmid-map-sequence-id-108468

    Enlarge

  12. pAV10.F6.loxP

    Plasmid
    #63201
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    63201-plasmid-map-sequence-id-267056

    Enlarge

  13. pAV10.F3.loxP

    Plasmid
    #63200
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63200-plasmid-map-sequence-id-104877

    Enlarge

  14. pAV10.K6.loxP

    Plasmid
    #63203
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63203-plasmid-map-sequence-id-104900

    Enlarge

  15. pAV10.K5.loxP

    Plasmid
    #63204
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63204-plasmid-map-sequence-id-104903

    Enlarge

  16. pAV10.HO3.loxP

    Plasmid
    #63205
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63205-plasmid-map-sequence-id-104905

    Enlarge

  17. pAV10.HO5.loxP

    Plasmid
    #63206
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63206-plasmid-map-sequence-id-104906

    Enlarge

  18. pAIP4_1EW4

    Plasmid
    #234180
    Purpose
    Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EW4 (cyaY Escherichia coli)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234180-plasmid-map-sequence-id-464145

    Enlarge

  19. pINEAPLE

    Plasmid
    #250295
    Purpose
    Big-IN Payload assembly vector by Golden Gate Cloning using Esp3I. Contains a bacterial green/white GFP-based screening system and a mammalian transient (backbone) BSD selection cassette.
    Depositor
    Matthew Maurano
    Article
    Ordoñez et al bioRxiv 2025.08.13.669251
    Insert
    SuperfolderGFP
    Use
    Synthetic Biology; Assembly vector for big-in pay…
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250295-plasmid-map-sequence-id-498935

    Enlarge

  20. pGA-blue

    Plasmid
    #196336
    Purpose
    Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196336-plasmid-map-sequence-id-393157

    Enlarge

Showing: 361 - 380 of 1063 results