Addgene

We narrowed to 1,297 results for: sds

Showing: 361 - 380 of 1297 results

Results per page:

Sort results by:

CSII-IDR2-humanTRIM5-FOS

Plasmid

#79066

Purpose

Express human TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(human)-FOS (TRIM5 Human)

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p6614 MSCV-IP N-HAonly PXMP3

Plasmid

#35012

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

PXMP3 (PEX2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only
pHLP712

Plasmid

#203741

Purpose

Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Expression

Mutation

Codon 74 changed from Phe to Ala (F74A)

Promoter

ACT1 (C. albicans)

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-OSF-TRIM5alpha(human)

Plasmid

#79034

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human) (TRIM5 Human)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAIP-hXRCC1pd-eGFP

Plasmid

#206033

Purpose

Mammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoter

Depositor

Primo Schaer

Article

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

hXRCC1

Use

Tags

eGFP

Expression

Mammalian

Mutation

S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

Promoter

CAG

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHLP701

Plasmid

#203743

Purpose

C-terminal tagging of target proteins with 3xV5-IAA17 degron

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

IAA17 auxin binding domain

Use

Pcr amplification template for gene tagging

Tags

3xV5

Expression

Mutation

Promoter

none

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p6618 MSCV-IP N-HAonly WHSC2

Plasmid

#35014

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

WHSC2 (NELFA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
p6592 MSCV-IP N-HAonly WWP2

Plasmid

#34905

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

WWP2 (WWP2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR-NLS

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet1-His-TEV-OTC_Ub

Plasmid

#172134

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p6642 MSCV-P C-FlagHA 6bE7-Kozak

Plasmid

#35020

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV6b E7 (E7 HPV 6b)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pET28a_speG_Cterm_His

Plasmid

#220483

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_PA4114_Cterm_His

Plasmid

#220484

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDY118A

Plasmid

#182958

Purpose

pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

Depositor

Shelley Copley

Article

Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

Inserts

Cas9
Gam, Beta, Exo
I-scel

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p6641 MSCV-P C-FlagHA 18E7-Kozak

Plasmid

#35019

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV18 E7 (E7 HPV 18)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

March 14, 2012

Availability

Academic Institutions and Nonprofits only
p6644 MSCV-P C-Flag HA 45E7-Kozak

Plasmid

#35135

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV45 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pCambia2300 - ProRUBY:RUBY-Citrine

Plasmid

#128796

Purpose

Fluorescent reporter for subcellular localisation and expression analysis of RUBY

Depositor

George Haughn

Article

Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

Insert

RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

ProRUBY

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p6656 MSCV-P C-Flag HA 25E7-Kozak

Plasmid

#35149

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV25 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6652 MSCV-P C-Flag HA 8E7-Kozak

Plasmid

#35145

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV8 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
p6653 MSCV-P C-Flag HA 92E7-Kozak

Plasmid

#35146

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV92 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only