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pLL3.7-EF-EYFP-YAP1_Y357F-PolyA

Plasmid

#112287

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoform

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Tyrosine 357 to Phenyalanine corresponding to tyr…

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A E252A

Plasmid

#244493

Purpose

Bacterial Expression of pH insensitive SHP2 mutant

Depositor

Katharine White

Publication

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine and Glutamate 25…

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A

Plasmid

#244492

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Publication

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 SHP2 E252A

Plasmid

#244491

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Publication

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Glutamate 252 to Alanine

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-deltaCKAR

Plasmid

#222429

Purpose

FRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.

Depositor

Alexandra Newton

Publication

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Plasma membrane delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-deltaCKAR

Plasmid

#222430

Purpose

FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

Depositor

Alexandra Newton

Publication

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Nucleus delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Mito(OM)-deltaCKAR

Plasmid

#222431

Purpose

FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

Depositor

Alexandra Newton

Publication

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Mitochondrial outer membrane delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Golgi-deltaCKAR

Plasmid

#222428

Purpose

FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

Depositor

Alexandra Newton

Publication

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Golgi delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper Retro GFP DGK zeta mouse

Plasmid

#224577

Purpose

Negative Control for downregulation of the expression of human DGK zeta in human cells

Depositor

Isabel Mérida

Publication

Torres-Ayuso et al Oncotarget. 2014 Oct 30;5(20):9710-26. d

Insert

sh RNAi Diacylglycerol kinase zeta mouse (Dgkz Mouse)

Use

RNAi and Retroviral

Promoter

H1

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper Retro GFP DGK alfa human

Plasmid

#224578

Purpose

To stably downregulate the expression of human DGK alfa in human cells

Depositor

Isabel Mérida

Publication

Torres-Ayuso et al Oncotarget. 2014 Oct 30;5(20):9710-26. d

Insert

sh RNAi Diacylglycerol kinase alfa human (DGKA Human)

Use

RNAi and Retroviral

Promoter

H1

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_R89A-PolyA

Plasmid

#112291

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Arginine 89 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_L91A-PolyA

Plasmid

#112292

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Leucine 91 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_F95A-PolyA

Plasmid

#112293

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Phenylalanine 95 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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SHIP2-ΔPS

Plasmid

#214901

Purpose

expression of the truncated variants of the SHIP2 without phosphatase domain

Depositor

Pavel Krejčí

Publication

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 delta aa 419-739 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 419-739aa

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PX459v2-ILK-gRNA 1-exon 1

Plasmid

#163320

Purpose

SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

Depositor

Val Brunton

Publication

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ILK gRNA 1_Exon1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
PX459v2-ILK-gRNA 2-exon 8

Plasmid

#163321

Purpose

SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

Depositor

Val Brunton

Publication

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ILK gRNA 2_Exon8

Use

CRISPR

Expression

Mammalian

Promoter

U6F

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG_FBXL7 (R310H)

Plasmid

#171849

Purpose

pcDNA3-FLAG_FBXL7 (R310H)

Depositor

Michele Pagano

Publication

Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

Insert

F-box and leucine rich repeat protein 7 (FBXL7 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Arginine 310 to Histidine

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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SHIP2-ΔPR1

Plasmid

#214902

Purpose

expression of the truncated variants of the SHIP2 without first proline-rich domain

Depositor

Pavel Krejčí

Publication

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 delta aa 123-410 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 123-410aa

Promoter

CMV

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-C2

Plasmid

#214906

Purpose

expression of the truncated variants of the SHIP2 that retains second proline-rich domain

Depositor

Pavel Krejčí

Publication

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 740-1189 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 1-739, 1190-1258aa

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-N2

Plasmid

#214904

Purpose

expression of the truncated variants of the SHIP2 that retains first proline-rich domain

Depositor

Pavel Krejčí

Publication

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 123-418 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 1-122, 419-1258aa

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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