We narrowed to 9,753 results for: try

1. pDONR221-ARF1-no stop

   Plasmid

   #67307

   Purpose

   hArf1 without stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF1 (ARF1 Human)
   

   Use

   Gateway entry vector

   Expression

   Bacterial

   Promoter

   None

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDONR221-ARF3-no stop

   Plasmid

   #67309

   Purpose

   hArf3 without stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF3 (ARF3 Human)
   

   Use

   Gateway entry vector

   Expression

   Bacterial

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pDONR221-ARF6-no stop

   Plasmid

   #67315

   Purpose

   hArf6 without stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Gateway entry vector

   Expression

   Bacterial

   Promoter

   None

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET30Xa-SBL1(160E,NoCys)

   Plasmid

   #66875

   Purpose

   Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term Histag

   Depositor

   Betty Gaffney

   Article

   Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

   Insert

   soybean lipoxygenase-1 (S160E) with all Cys to Ser mutations
   

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   S160E; changed 4 (all) cysteines to serine

   Promoter

   T7lac

   Available Since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mutpRL-TK 1x perfect to bulged siRNA

   Plasmid

   #40765

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA perfect to bulged target site
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. mutpRL-TK 1x perfect to seed siRNA

   Plasmid

   #40766

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA perfect to seed target site
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

7. mutpRL-TK 1x perfect to seed plus 13-16 siRNA

   Plasmid

   #40767

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA perfect to seed plus 13-16 target site
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pEN_hU6miR-Gb2-K

   Plasmid

   #25759

   Purpose

   Entry vector with human U6 promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Gb2 miR-shRNA (Gnb2 Mouse)
   

   Use

   Entry vector

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pA-CBh.TP-CNOT8-V5H6.MCh.Puro

   Plasmid

   #209947

   Purpose

   In mammalian cells expresses TP fused to a CNOT8 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

   Depositor

   Kevin Weeks

   Article

   Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

   Inserts

   CCR4-NOT transcription complex subunit 8 (CNOT8 Human)
   mCherry fluorescent protein fused to puromycin resistance marker
   

   Tags

   TP (MS2 coat protein) and V5 epitope with H6 tag

   Expression

   Mammalian

   Promoter

   CBh and CMV

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104140

    Purpose

    Expresses TREAT-5'TOP reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

    Expression

    Mammalian

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIV-IL13-CD8aStalk-PDGFR

    Plasmid

    #240242

    Purpose

    Lentiviral transfer plasmid encoding IL13 with CD8a stalk and PDGFR TM domain

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    IL-13 (IL13 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV14-3X-Flag-SARS-CoV-2 S

    Plasmid

    #145780

    Purpose

    Over expression of SARS-CoV-2 S (spike) in mammalian cell line

    Depositor

    Zhaohui Qian

    Article

    Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:

    Insert

    SARS-CoV-2 S (S SARS CoV 2)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Codon optimized and C-terminal 19 amino acids del…

    Promoter

    CMV

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pHIV-IL13-IgG4hinge-PDGFR

    Plasmid

    #240245

    Purpose

    Lentiviral transfer plasmid encoding IL13 with IgG4 hinge and PDGFR TM domain

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    IL-13 (IL13 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCF140_Gag-SpyCas9-NLS

    Plasmid

    #225959

    Purpose

    CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-Gag-SpyCas9-NLS
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. 139H2_HC

    Plasmid

    #206201

    Purpose

    For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.

    Depositor

    Joost Snijder

    Article

    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

    Insert

    anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)
    

    Tags

    -AAAHHHHHHHH

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ACE2

    Plasmid

    #164219

    Purpose

    Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Mutation

    silent mutation to remove internal EcoRI site (GA…

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR1A-TAZ S89A

    Plasmid

    #179911

    Purpose

    Entry vector of TAZ S89A for gateway coloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Insert

    WW Domain Containing Transcription Regulator 1 (WWTR1 Human)
    

    Use

    Entry vector

    Tags

    3X Flag-tag

    Mutation

    Serine 89 mutated to Alanine

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR1A FKBP12F36V-OGT

    Plasmid

    #154287

    Purpose

    Entry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc Transferase)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x HA and FKBP12(F36V)

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-GST-BcPC-PLC

    Plasmid

    #224255

    Purpose

    Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

    Depositor

    Chiaki Murakami

    Article

    Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

    Insert

    Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
    

    Tags

    GST and HRV-3C (LEVLFQGP) site

    Expression

    Bacterial

    Mutation

    The gene of Bacillus cereus ATCC 14579 was used f…

    Promoter

    lac promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid

    #127177

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    Flag- HA- Tandem Epitopes

    Promoter

    None

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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