We narrowed to 4,100 results for: biorxiv

1. pET3a.H2B-K120C

   Plasmid

   #224706

   Purpose

   Expresses Human H2B K120C mutant in bacterial cells for fluorescent labelling of histone octamers

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   H2B-K120C (H2B Human)
   

   Expression

   Bacterial

   Mutation

   K120C

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5E-EFS (EF1a core) (JDW 1319)

   Plasmid

   #224474

   Purpose

   Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EFS promoter
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

   Plasmid

   #224496

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

   Plasmid

   #224505

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-mODC-LexOP-35SCaMV
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

   Plasmid

   #224506

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-oMDC-LexOP-c-Fos
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

   Plasmid

   #224512

   Purpose

   A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xHA-BirA-T2A-mCherry-Caax
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

   Plasmid

   #224522

   Purpose

   A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
   

   Use

   Cre/Lox

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E 5X C120 c-fos min pro (JDW 1241)

   Plasmid

   #224544

   Purpose

   A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   5x-C120-c-Fos promoter
   

   Use

   Gateway cloning

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)

   Plasmid

   #194226

   Purpose

   Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)

    Plasmid

    #194233

    Purpose

    Mammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domain

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    CD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258 (S Synthetic)
    

    Use

    Synthetic Biology

    Promoter

    None

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBK1297-AAV-EFSNC-dCjCas9-HP1b(79-173)

    Plasmid

    #223142

    Purpose

    Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1b (CBX1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 79-173

    Promoter

    EF1a

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBK1876-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

    Plasmid

    #223162

    Purpose

    Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

    Plasmid

    #223143

    Purpose

    Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1b (CBX1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 111-173

    Promoter

    EF1a

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBK1308-AAV-EFSNC-dSaCas9-HP1bNH(111-173)

    Plasmid

    #223153

    Purpose

    Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1b (CBX1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 111-173

    Promoter

    EF1a

    Available Since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)

    Plasmid

    #223152

    Purpose

    Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1b (CBX1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 79-173

    Promoter

    EF1a

    Available Since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK1861-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

    Plasmid

    #223161

    Purpose

    Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

    Plasmid

    #223166

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330_DAZL_cterm2

    Plasmid

    #222919

    Purpose

    Cas9/sgRNA plasmid for targeting DAZL

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, DAZL sgRNA 2 (DAZL Synthetic, Human)
    

    Use

    CRISPR

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330_DAZL_cterm3

    Plasmid

    #222920

    Purpose

    Cas9/sgRNA plasmid for targeting DAZL

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, DAZL sgRNA 3 (DAZL Synthetic, Human)
    

    Use

    CRISPR

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330_TFAP2C_cterm

    Plasmid

    #222916

    Purpose

    Cas9/sgRNA plasmid for targeting TFAP2C

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, TFAP2C sgRNA (TFAP2C Synthetic, Human)
    

    Use

    CRISPR

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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