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We narrowed to 9,876 results for: ef1;

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Showing: 3801 - 3820 of 9876 results

  1. CROPseq-Puro-2xMS2

    Plasmid
    #212627
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212627-plasmid-map-sequence-id-420278

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  2. CROPseq-Puro-12xMS2

    Plasmid
    #212628
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212628-plasmid-map-sequence-id-422312

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  3. CROPseq-Puro-4xMS2

    Plasmid
    #212626
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212626-plasmid-map-sequence-id-420274

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  4. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a core promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219823-plasmid-map-sequence-id-441127

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  5. pWR23

    Plasmid
    #202770
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coKanMX
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202770-plasmid-map-sequence-id-417278

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  6. pWR10

    Plasmid
    #202710
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coShBle
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202710-plasmid-map-sequence-id-417297

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  7. pWR9

    Plasmid
    #202645
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202645-plasmid-map-sequence-id-409735

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  8. pWR54

    Plasmid
    #203330
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    203330-plasmid-map-sequence-id-417329

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  9. pWR24

    Plasmid
    #202771
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coNAT
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    202771-plasmid-map-sequence-id-409762

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  10. pWR16

    Plasmid
    #202711
    Purpose
    integrating bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    URA3 and flanking sequence
    Expression
    Yeast
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    202711-plasmid-map-sequence-id-409745

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  11. pILGFP8EFA

    Plasmid
    #185854
    Purpose
    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    Expression
    Yeast
    Mutation
    DHFR P66L
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185854-plasmid-map-sequence-id-370766

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  13. ARB287d

    Plasmid
    #124018
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with a spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-spacer_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124018-plasmid-map-sequence-id-252457

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  14. ARB287e

    Plasmid
    #124019
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with a multicistronic spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-spacermulticistronic_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124019-plasmid-map-sequence-id-252460

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  15. JPF0464

    Plasmid
    #124035
    Purpose
    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a human non-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    pHR_pEF1a-iRFP713_non targeting sgRNA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124035-plasmid-map-sequence-id-252506

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  16. JPF0465

    Plasmid
    #124036
    Purpose
    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a mScarlet-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    pHR_pEF1a-iRFP713_mScarlet sgRNA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124036-plasmid-map-sequence-id-252507

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  17. JPF0466

    Plasmid
    #124037
    Purpose
    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a tagBFP-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    pHR_pEF1a-iRFP713_tagBFP sgRNA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124037-plasmid-map-sequence-id-262668

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  18. JPF0468

    Plasmid
    #124038
    Purpose
    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    pHR_pEF1a-iRFP713_mScarletsgRNA_tagBFP sgRNA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124038-plasmid-map-sequence-id-252546

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  19. ARB287a

    Plasmid
    #124015
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with a BgpPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124015-plasmid-map-sequence-id-252435

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  20. ARB287b

    Plasmid
    #124016
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with a SV40 3' UTR and a mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-SV40pA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124016-plasmid-map-sequence-id-252439

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Showing: 3801 - 3820 of 9876 results