We narrowed to 9,957 results for: pET
-
Plasmid#172096PurposeExpresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteriaDepositorInsertGaussia Luciferase - iLID
ExpressionBacterialMutationGluc Mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-Gluc-EL222-his
Plasmid#172097PurposeExpresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteriaDepositorInsertGaussia Luciferase - EL222
ExpressionBacterialMutationGluc mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-eReader
Plasmid#135090PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered x3
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-6His-DmBelle_1-694_B
Plasmid#145951PurposeBacterial Expression of DmBelle_1-694DepositorAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT-L713F
Plasmid#173944PurposeBacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutationDepositorAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub(T/A)-YPet-NES
Plasmid#138208PurposeEncodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub(T/A)-YPet-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
human TLNRD1-F250D pET151
Plasmid#159385PurposeExpresses human TLNRD1 F250D mutant in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)
TagsHis-tag, TEV cleavage siteExpressionBacterialMutationChanged Phenylalanine 250 to Aspartic Acid (F250D)PromoterT7Available sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
Tags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-Strep-SsOGT-H5-His
Plasmid#167277PurposeOverexpression of a sulfolobal equivalent of SNAP-tag in E. coliDepositorInsertSsOGT-H5
TagsHis x10 and Strep-tagExpressionBacterialPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mCherry-TEAD4-IDR
Plasmid#166442PurposeBacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion proteinDepositorInsertmCherry, TEAD4-IDR (TEAD4 Human)
ExpressionBacterialAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-HisDAD2 cPGFP(G164)
Plasmid#165997PurposeExpression of coding sequence in Escherichia coliDepositorInsertHisDAD2 cPGFP(G164)
Tags6HisExpressionBacterialPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(G164)
Plasmid#165993PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(G164)
ExpressionBacterialPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-YPET-C1-WPRE
Plasmid#159954Purposeuntargeted markerDepositorPublicationInsertYPET-C1
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-C138A
Plasmid#133911PurposeBacterial expression of His6-Ubc9-C138ADepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
TagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 138 to AlaninePromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22-Strep-TEV-Nsp10
Plasmid#154958PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coliDepositorAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET45b-tsr(B/X) (6X-His-Dm-Cofilin)
Plasmid#128278PurposeExpresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell cultureDepositorInserttwinstar (tsr) (tsr Fly)
Tags6X Histidine tagExpressionBacterialMutationMet1 changed to Pro1; Cys77 silent basepair mutat…PromoterT7Available sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)
Plasmid#127636PurposeEncodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)DepositorInsertmTurquoiuse2-Tat, YPet-PCP
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW YPET-T2A-mTurquoise2
Plasmid#118450PurposeProduces equimolar expression of YPET and mTq2DepositorInsertYPET-T2A-mTurquoise2
ExpressionYeastAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymYPET-T2A-mTurquoise2
Plasmid#118444PurposeProduces equimolar expression of ymYPET and mTq2DepositorInsertymYPET-T2A-mTurquoise2
ExpressionYeastMutationYPET A206K, F208S, E232L, N235DAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/G51PEKN
Plasmid#118583PurposeBacterial expression of E.coli DHFR N23PP and G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationN23 mutated to P23, G51 mutated to P51, a Pro res…PromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only