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pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

Plasmid

#61582

Purpose

Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne2 (OTUD7A Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Isoform 2. Deleted aa 463-933.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLVU-coHPDL WT

Plasmid

#174128

Purpose

Expresses wild type codon-optimized HPDL in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Codon optimized

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-NLRP1 codon-optimized

Plasmid

#166833

Purpose

Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

HIS

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-DAXX-ΔD/E-His

Plasmid

#179618

Purpose

Express GST-DAXX-ΔDE-His in bacteria

Depositor

Xiaolu Yang

Article

Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

Insert

death domain associated protein (DAXX Human)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 424-495

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-DAXX-D/E-His

Plasmid

#179619

Purpose

Express DAXX D/E region in bacteria

Depositor

Xiaolu Yang

Article

Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

Insert

death domain associated protein (DAXX Human)

Tags

GST, His, and TEV

Expression

Bacterial

Mutation

amino acids 414-505 of DAXX

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG

Plasmid

#166803

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF1821

Plasmid

#143158

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF138 (ZNF138 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S

Plasmid

#166794

Purpose

Mammalian cell expression vector for Flag-tagged DPP9S

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-DAXX-His

Plasmid

#175781

Purpose

DAXX protein for baculovirus expression system

Depositor

Xiaolu Yang

Article

Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

Insert

death domain associated protein (DAXX Human)

Tags

GST and TEV

Expression

Insect

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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VRK1-dTag-puro

Plasmid

#199651

Purpose

Expresses VRK1 C-term. Tagged with dTAG

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK1 (VRK1 Human)

Use

Lentiviral

Tags

dTAG

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCR1163

Plasmid

#111099

Purpose

Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCD2 gRNA (FANCD2 Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317-VRK1-K179E

Plasmid

#199644

Purpose

Expresses kinase-inactive VRK1 (K179E)

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK1 (VRK1 Human)

Use

Lentiviral

Mutation

K179E

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-FAM208A ts2

Plasmid

#115872

Purpose

FAM208a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

FAM208a shRNA (C3orf63 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-mSTING (N153S)

Plasmid

#214158

Purpose

Transient expression of gain-of-function STING mutant

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (N153S) (Sting1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

N153S

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgVRK1 #4-Blast

Plasmid

#199647

Purpose

Expresses Cas9 and sgRNA guide targeting VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Plasmid

#172514

Purpose

Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Use

AAV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SETDB1 ts1

Plasmid

#115859

Purpose

SETDB1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SETDB1 shRNA (SETDB1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3xVHH05-DsRed

Plasmid

#171580

Purpose

For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3xVHH05 (UBE2J1 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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DRH323: pAAV_hSyn-QuasAr2

Plasmid

#107705

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr2

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-FAM208A ts3

Plasmid

#115873

Purpose

FAM208a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

FAM208a shRNA (C3orf63 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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