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pDRF1-GW YPET-T2A-mTurquoise2

Plasmid

#118450

Purpose

Produces equimolar expression of YPET and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

YPET-T2A-mTurquoise2

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMM112

Plasmid

#127229

Purpose

35S::IPT::pinII terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

IPT

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pRSF-KpAGO207-1251

Plasmid

#117854

Purpose

Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

Depositor

Kotaro Nakanishi, Dinshaw Patel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Argonaute

Tags

6xHis-SUMO encoded in vector

Expression

Bacterial

Mutation

missing first 206 amino acid residues

Promoter

T7

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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TRF2ΔBΔM/pENTR

Plasmid

#19797

Depositor

Mark Mattson

Article

van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

Insert

TRF2 (TERF2 Human)

Use

Entry vector

Mutation

deleted amino acids 1-44 and 450-500

Available Since

April 15, 2009

Availability

Academic Institutions and Nonprofits only
pFlagCMV2-14-3-3sigma

Plasmid

#12453

Depositor

Dong-Er Zhang

Article

Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

Insert

14-3-3 sigma (SFN Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pFUGW NLS-Cre

Plasmid

#183425

Purpose

Lentiviral Cre vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

NLS-Cre

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iPASnFR-H-mScarlet3-3xFLAG-Rab5

Plasmid

#225243

Purpose

Mammalian expression of iPASnFR-H fused to Rab5

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Ube2v1

Plasmid

#31429

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2V1 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5

Plasmid

#225244

Purpose

Mammalian expression of iPASnFR-L fused to Rab5

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDEW8

Plasmid

#84238

Purpose

Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae

Depositor

David Weinberg

Article

DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1

Insert

HA-mRuby-P2A-eGFP

Tags

HA-mRuby and eGFP

Expression

Yeast

Promoter

GPD

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ytdTomato

Plasmid

#118456

Purpose

Overexpression of yeast codon-optimized tdTomato

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2_LSEC:Cas9green; erbb2_gRNA171

Plasmid

#199338

Purpose

pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

LSEC
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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Ube2d1

Plasmid

#31409

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2D1 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
Ube2b

Plasmid

#31408

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2B Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pOC1 cloning template vector

Plasmid

#183420

Purpose

CreON knock-in vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 cloning template vector

Plasmid

#183421

Purpose

CreOFF knock-in vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMM107

Plasmid

#127227

Purpose

35S::IPT::pinII terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

IPT

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Paxillin

Plasmid

#186142

Purpose

For expresseion of recombinant paxillin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

Paxillin C1997A (silent mutation for cloning)

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymVenus

Plasmid

#118454

Purpose

Overexpression of yeast codon-optimized mVenus

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRS405-LEU2-TEF-KpolDcr1v2

Plasmid

#85193

Purpose

Integrating plasmid, K. polysporus Dcr1 under TEF promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Dcr1

Use

Integrating, shuttle vector

Expression

Yeast

Promoter

TEF

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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