We narrowed to 7,306 results for: GFP expression plasmids

1. pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

   Plasmid

   #84485

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1α, 1.1 kb long (short version)

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]

   Plasmid

   #128588

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1α (1.1 kb short version)

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP

   Plasmid

   #194016

   Purpose

   Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

   Depositor

   Larry Young

   Article

   Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

   Inserts

   sgRNA(Oxtr.2)
   eGFP
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   cmb and u6

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter

   Plasmid

   #172598

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-D842V (PDGFRA Synthetic, Human)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-D842V

   Promoter

   CMV

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-GFP-Puro_TAL1-long

   Plasmid

   #210642

   Purpose

   Overexpression of TAL1-long

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   TAL1-long (TAL1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CMV-GFP-Puro_TAL1-short

   Plasmid

   #210641

   Purpose

   Overexpression of TAL1-short

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   TAL1-short (TAL1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

   Plasmid

   #121966

   Purpose

   Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   superfold GFP-GB1-NLS-iLID

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

   Plasmid

   #127332

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159672

   Purpose

   Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   βTub-NLS-hSpCas9-T2A-GFP
   

   Tags

   T2A-GFP

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159671

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    ExuL-NLS-hSpCas9-T2A-GFP
    

    Tags

    T2A-GFP

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500

    Plasmid

    #92227

    Purpose

    Expression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    deGFP Y35TAG D193TAG
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residues Y35 D193 were mutated into TAG (Amber) s…

    Promoter

    p70a

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. BCL6-GFP (1-275AA) in Cilantro

    Plasmid

    #169932

    Purpose

    Overexpression of 1-275AA BCL6-GFP fusion

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

    Insert

    BCL6 1-275AA (BCL6 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Mutation

    160R>C

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

    Plasmid

    #211760

    Purpose

    Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

    Depositor

    Glenn Yiu

    Article

    Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    gRNA1 and gRNA2 targeting VEGF-A (VEGFA Mouse, Human, M. mulatta (rhesus macaque))
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-Flag-ER-Hoxb8-IRES-GFP

    Plasmid

    #222292

    Purpose

    Less commonly used plasmid for generating ER-Hoxb8 conditionally immortalized myeloid cell lines.

    Depositor

    Mark Kamps, David Sykes

    Article

    Wang et al Nat Methods. 2006 Apr;3(4):287-93.

    Insert

    ER-Hoxb8 (ESR1, Hoxb8 Mouse, Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    The estrogen receptor hormone binding domain cont…

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKII-Jaws-KGC-GFP-ER2

    Plasmid

    #65015

    Purpose

    AAV-mediated expression of Jaws under the CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CaMKII

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-GSDMD-eGFP-SV40p-BlasR

    Plasmid

    #228254

    Purpose

    pLenti-CMV-GSDMD-eGFP-SV40p-BlasR plasmid for ectopic expression of human full-length GSDMD fusion with GFP

    Depositor

    Judy Lieberman

    Article

    Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hsyn-Jaws-KGC-GFP-ER2

    Plasmid

    #65014

    Purpose

    AAV-mediated expression of Jaws under the human synapsin promoter

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Has Service

    AAV Retrograde, AAV5, and AAV8

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    human synapsin

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1α1.1-Jaws-KGC-GFP-ER2

    Plasmid

    #153537

    Purpose

    AAV-mediated expression of Jaws-GFP-ER2 under the EF1α promoter (1.1kb short version).

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    EF1α1.1

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUW-ubiquitin-EphB2-SV40-GFP (B2G)

    Plasmid

    #65442

    Purpose

    Expression of mouse EPHB2 and GFP in mammalian cells

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    EPHB2 (Ephb2 Mouse)
    

    Use

    Lentiviral

    Tags

    SV40-EGFP

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    UBQ

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. anti-GFP scFv [N86/38] with sortase tag

    Plasmid

    #204419

    Purpose

    Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting GFP (Aequorea victoria)
    

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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