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pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

Plasmid

#182009

Purpose

CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Publication

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

IgKchL_HA_STf_myc_PDGFRtmb

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL

Plasmid

#182012

Purpose

CMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell lines

Depositor

Kai Johnsson

Publication

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

CalR-HaloTag7-P30-SNAP-tag-KDEL

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-HygroR-T2A-TetOn3G

Plasmid

#179887

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Publication

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

Hygromycin resistance

Expression

Bacterial and Mammalian

Promoter

EF1a

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEF Doc2alpha WT GFP

Plasmid

#128819

Purpose

To visualize Doc2alpha WT in PC12 cells

Depositor

Edwin Chapman

Publication

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Tags

EGFP

Expression

Mammalian

Promoter

EF-1alpha

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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TB:7xE-Box:MiniP

Plasmid

#133880

Purpose

7 copies of the E-box motif

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

7 copies of the E-box motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX-TangoVL-lexA

Plasmid

#138406

Purpose

RMCE vector for generating C-terminal Tango fusion

Depositor

Shu Kondo

Publication

Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

Insert

V2 tail-TEVcs(ENLYFQL)-LexAp65

Expression

Insect

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-DHFR-myc

Plasmid

#118852

Purpose

For fluorophore translocation assay and inducible tagging with zapalog.

Depositor

Thomas Schwarz

Publication

Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

Type

Empty backbone

Tags

DHFR, Myc, and YFP

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

Plasmid

#114726

Purpose

dCas9-VP160 expression in mammalian cells

Depositor

Martine Roussel

Publication

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

dCas9-VP160

Expression

Mammalian

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRetro-XT-PAK2-CFP(puro)-L107F

Plasmid

#81180

Purpose

Retroviral vector for expression of kinase active PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Publication

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Mutation

L107F

Promoter

P-tight TRE

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pET28a-GGG-HIS-CBM-Xmod-DocIII

Plasmid

#91698

Purpose

CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

Depositor

Hermann Gaub

Publication

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-HIS-CBM-Xmod-DocIII

Tags

HIS-Tag and Sortase-Tag

Expression

Bacterial

Mutation

Cysteine deletion in CBM C63S

Promoter

T7

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-deltaR8.2(A14C,E45C,A92E)

Plasmid

#79048

Purpose

mammalian expression, lentiviral packaging

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-deltaR8.2(A14C,E45C)

Plasmid

#79047

Purpose

mammalian expression, lentiviral packaging

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEBB COMMD8 Flag

Plasmid

#74900

Purpose

Express COMMD8 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Publication

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD8 (COMMD8 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD7 Flag

Plasmid

#74899

Purpose

Express COMMD7 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Publication

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD7 (COMMD7 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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Kohinoor/pRSETb

Plasmid

#67770

Purpose

Kohinoor for bacterial expression

Depositor

Takeharu Nagai

Publication

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSi

Plasmid

#67884

Purpose

Derivative of pSiM24 plant expression vector without the M24 promoter

Depositor

Indu Maiti

Publication

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Type

Empty backbone

Expression

Plant

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-Caveolin3DGV

Plasmid

#67886

Purpose

Fluorescently tagged Caveolin-3 truncation mutant for expression in mammalian cells

Depositor

Rob Parton

Publication

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the first 53 amino acids of Caveolin3

Promoter

CMV

Available since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only
mTagRFP-T-Fibrillarin-7

Plasmid

#58016

Purpose

Localization: Nucleoli, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Fibrillarin (FBL Human)

Tags

mTagRFP

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
DHFR

Plasmid

#64200

Purpose

Expression of E. coli DHFR

Depositor

Vern Schramm

Publication

Wang et al J Am Chem Soc. 2014 Jun 11;136(23):8333-

Insert

Dihydrofolate reductase

Tags

none

Expression

Bacterial

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pSB4A5 JQ929581

Plasmid

#38168

Depositor

Drew Endy

Publication

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

mKate2
Superfolder GFP

Use

Synthetic Biology; Biobrick cloning vector

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only