We narrowed to 5,308 results for: pcDNA3.1+

1. Vinculin-Clover-CC-S4-mRuby2

   Plasmid

   #233235

   Purpose

   Vinculin FRET tension sensor with coiled-coil S4 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S4-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Vinculin-Clover-CC-S2-mRuby2

   Plasmid

   #233234

   Purpose

   Vinculin FRET tension sensor with coiled-coil S2 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S2-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9

   Plasmid

   #231907

   Purpose

   For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   EGFP-P2A-dTomato-T2A-TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Const.4 EGFP-IRES-Luc-PS9

   Plasmid

   #231908

   Purpose

   For packaging EGFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   EGFP-IRES-Luc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Vh-Clover-CC-S2-mRuby2-Vt

   Plasmid

   #233227

   Purpose

   Vinculin FRET tension sensor with coiled-coil S2 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S2-mRuby2-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Vh-Clover-CC-M2-mRuby2-Vt

   Plasmid

   #233231

   Purpose

   Vinculin FRET tension sensor with coiled-coil M2 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-M2-mRuby2-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Vinculin-Clover-CC-Mad-mRuby2

   Plasmid

   #233239

   Purpose

   Vinculin FRET tension sensor with coiled-coil Mad domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-Mad-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Vh-Clover-CC-S1-mRuby2-Vt

   Plasmid

   #233230

   Purpose

   Vinculin FRET tension sensor with coiled-coil S1 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S1-mRuby2-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Vh-Clover-CC-S0-mRuby2-Vt

   Plasmid

   #233226

   Purpose

   Vinculin FRET tension sensor with coiled-coil S0 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S0-mRuby2-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Vh-Clover-CC-S4-mRuby2-Vt

    Plasmid

    #233228

    Purpose

    Vinculin FRET tension sensor with coiled-coil S4 domain

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    Vh-Clover-CC-S4-mRuby2-Vt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Vh-Clover-CC-S7-mRuby2-Vt

    Plasmid

    #233229

    Purpose

    Vinculin FRET tension sensor with coiled-coil S7 domain

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    Vh-Clover-CC-S7-mRuby2-Vt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Vh-Clover-CC-Mad-mRuby2-Vt

    Plasmid

    #233232

    Purpose

    Vinculin FRET tension sensor with coiled-coil Mad domain

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    Vh-Clover-CC-Mad-mRuby2-Vt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Vinculin-Clover-CC-S0-mRuby2

    Plasmid

    #233233

    Purpose

    Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    Vinculin-Clover-CC-S0-mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-Sga-Cas9

    Plasmid

    #227005

    Purpose

    Mammalian expression of Nuclease active S. gallolyticus Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-Sin-Cas9

    Plasmid

    #227006

    Purpose

    Mammalian expression of Nuclease active S. iniae Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-Spa-Cas9

    Plasmid

    #227007

    Purpose

    Mammalian expression of Nuclease active S. parasanguinis Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-Sub-Cas9

    Plasmid

    #227008

    Purpose

    Mammalian expression of Nuclease active S. uberis Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mproSAAS (21 kDa)

    Plasmid

    #231634

    Purpose

    Mammalian expression of the N-terminal domain of the mouse chaperone protein proSAAS-(1-180)

    Depositor

    Iris Lindberg

    Article

    Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

    Insert

    Pcsk1N (Pcsk1n Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of C-terminus (aa181-225)

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SARS-CoV spike all5KO mutant

    Plasmid

    #231921

    Purpose

    For expression of SARS-CoV spike protein with glycan deletion at N1080, N1116, N1140, N1155, N1176 by implementing Asn-to-Gln mutation

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

    Insert

    spike all5KO (S SARS-CoV)
    

    Use

    Tags

    C9 and CD5 signal peptide

    Expression

    Mammalian

    Mutation

    N1080Q, N1116Q, N1140Q, N1155Q, N1176Q

    Promoter

    CMV

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTBL435

    Plasmid

    #226628

    Purpose

    pcDNA-dTdT

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Insert

    catalytically deactivated TdT (DNTT Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    D343E, D345E

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge