We narrowed to 5,543 results for: pcDNA3.1+

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hLOXL2-S300P

Plasmid

#200061

Purpose

LOXL2 mutant

Depositor

Lakshmi Santhanam

Article

Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

Insert

human lysyl oxidase like 2 (LOXL2 Human)

Expression

Mammalian

Mutation

S300P mutation

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre_60.F2

Plasmid

#60208

Purpose

Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Synthetic, Human, Bacteriophage P1)

Tags

FRAP and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 2021-2112 of FRAP with a T2098L mutat…

Promoter

CMV

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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YFP Rab4

Plasmid

#99018

Purpose

Localization and visualization of trafficking protein Rab4

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Rab4 (Rab4 Synthetic, Fly)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

Plasmid

#169531

Purpose

Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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Venus wGBD

Plasmid

#85222

Purpose

Venus tagged Cdc-42 sensor

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Insert

wGBD (WASL Human)

Tags

Venus

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(WT)

Plasmid

#137826

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(WT)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTS2396-Tier1-PhCMV-4xCOX8_Ypet

Plasmid

#169534

Purpose

Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven miochondria-localized yellow fluorescent protein Ypet

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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Bongwoori

Plasmid

#63720

Purpose

Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D

Depositor

Bradley Baker

Article

Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

Insert

Bongwoori

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

Plasmid

#188992

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

K109R, P245A, G328S, G381V, G429D

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-optoB2-LYY-YFP

Plasmid

#75271

Purpose

G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

G-protein independent version of optoB2

Tags

eYFP

Expression

Mammalian

Mutation

point mutations at L72F,Y136G,Y224A

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-NLS-ZapCmR2

Plasmid

#59012

Purpose

Nuclear zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NLS-ZapCmR2 genetically encoded Zn(II) sensor

Tags

SV40 NLS

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCmR2

Plasmid

#59015

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK2 Y261A

Plasmid

#8977

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

Y261A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
mGFAP-Halo

Plasmid

#169474

Purpose

the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

GFAP (Gfap Mouse)

Tags

HaloTag

Expression

Mammalian

Mutation

Changed R 236 to H

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgH (Merlin)

Plasmid

#136448

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-PH-dL5**

Plasmid

#96947

Purpose

Expresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Tags

EGFP and dL5

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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Dr-EGFR

Plasmid

#184004

Purpose

Dr-EGFR expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

Insert

Dr-EGFR (EGFR Human)

Tags

Deinococcus radiodurans bacterial phytochrome

Expression

Mammalian

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 L232A

Plasmid

#8976

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

L232A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pTS2410-Tier1-PhCMV-Myr_mTagBFP2

Plasmid

#169535

Purpose

Tier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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Lmajor_gp63_10_0460_E265A

Plasmid

#171641

Purpose

Expression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al Front Cell Infect Microbiol. 2019 Aug 7;

Insert

LmjF.10.0460 E265A

Tags

Myc-His

Expression

Mammalian

Mutation

E265A

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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