We narrowed to 32,965 results for: uros

1. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

   Plasmid

   #183409

   Purpose

   piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

   Plasmid

   #132398

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

   Plasmid

   #214875

   Purpose

   Empty lentiviral backbone for guide array cloning

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

   Plasmid

   #176851

   Purpose

   lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   hYAP1-5SA (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S61A, S109A, S127A, S164A, S381A (phosphorylation…

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

   Plasmid

   #209079

   Purpose

   Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   sfGFP
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE4G

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216246

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgAAVS1.5
   

   Use

   CRISPR; Human safe harbor locus (a avs1) site-spe…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

   Plasmid

   #132411

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dRfxCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   R239A; H244A; R858A; H863A

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag

   Plasmid

   #132402

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

   Plasmid

   #138148

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NES

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro

    Plasmid

    #141367

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

    Plasmid

    #132404

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

    Plasmid

    #208377

    Purpose

    Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

    Depositor

    Daniel Hawiger

    Article

    Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human CMV

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

    Plasmid

    #177203

    Purpose

    encoding stblHIF under inducible promoter

    Depositor

    Markus Covert

    Article

    Kudo et al bioRxiv (2022)

    Insert

    stblHIF
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P402A/P577G/N813A

    Promoter

    TRE

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid

    #123363

    Purpose

    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    EGFP-NLS
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

    Plasmid

    #141383

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT

    Plasmid

    #207832

    Purpose

    telomere specific nuclease to create double-strand breaks at telomeres

    Depositor

    Roger Greenberg

    Article

    Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag, DD, ER, mCherry and FokI

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132397

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1058A

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

    Plasmid

    #221235

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor

    Plasmid

    #184951

    Purpose

    Donor for both PiggyBac and Cp36 Recombinase

    Depositor

    Ami Bhatt, Patrick Hsu, Lacra Bintu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    PuroR, P2A, mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132400

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPguCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H151A; H1121A

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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