We narrowed to 7,297 results for: ef1a

1. Lenti_Neo_ZFP64_N_3XFLAG

   Plasmid

   #118700

   Purpose

   Express 3xflag tagged ZFP64

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Insert

   ZFP64 (ZFP64 Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG was fused to the N terminal of ZFP64 cDNA

   Expression

   Mutation

   Promoter

   EF1A

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCh-665

   Plasmid

   #92254

   Purpose

   Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   TNRC6C CED (TNRC6C Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   C-terminal effector domain (CED): aa 1369-1690 wi…

   Promoter

   EF1A

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCh-1522

   Plasmid

   #82428

   Purpose

   Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   nvGW182 CED 11W11A
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   amino acids 1-1158 of nvGW182 were deleted and Tr…

   Promoter

   EF1A

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCh-1539

   Plasmid

   #82432

   Purpose

   Plasmid for expression of GST-tagged human-Nematostella GW182 chimera in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   hs-nvGW182 chimera
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

   Promoter

   EF1A

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SL542

   Plasmid

   #49951

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD RH-8 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

6. SL544

   Plasmid

   #49970

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD RH-10 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

7. SL545

   Plasmid

   #49953

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD RH-11 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

8. SL543

   Plasmid

   #49952

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD RH-9 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

9. SL541

   Plasmid

   #49950

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD RH-7 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

10. SL540

    Plasmid

    #49949

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-6 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pLKO5d.EFS.SpCas9.P2A.BSD

    Plasmid

    #57821

    Purpose

    Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    EFS (EEF1A1 Human)
    P2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO5d.EFS.SpCas9.P2A.PAC

    Plasmid

    #58329

    Purpose

    Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    EFS (EEF1A1 Human)
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] (Recombinant Antibody)

    Antibody

    #211756-rAb

    Purpose

    Recombinant mouse monoclonal Anti-SARS-CoV-2 nucleocapsid protein antibody

    Depositor

    Brian Geiss

    Article

    Terry et al Virology. 2021 Jun;558:28-37. doi: 10.10

    Recommended Applications

    Western Blot

    Reactivity

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Not available to purchase

    Available since

    March 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

14. Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] (Recombinant Antibody)

    Antibody

    #211757-rAb

    Purpose

    Recombinant mouse monoclonal Anti-SARS-CoV-2 nucleocapsid protein antibody

    Depositor

    Brian Geiss

    Article

    Terry et al Virology. 2021 Jun;558:28-37. doi: 10.10

    Recommended Applications

    Western Blot

    Reactivity

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Not available to purchase

    Available since

    March 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

15. JPF0535a

    Plasmid

    #124042

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. ARB365

    Plasmid

    #124048

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ARB366

    Plasmid

    #124049

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ARB367

    Plasmid

    #124050

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF2058

    Plasmid

    #141898

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NKX2-2 (NKX2-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF2532

    Plasmid

    #142320

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX3 (SOX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge