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SARS-CoV-2 RBD Wuhan-Hu-1 SSM Library

Pooled Library

#1000000182

Depositor

Jesse Bloom

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only
pTKA001

Plasmid

#164577

Purpose

Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

Depositor

Robert Kirchdoerfer

Article

Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

Insert

nsp7 (ORF1ab )

Tags

6xHis, Ek, TEV

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL8B

Plasmid

#67467

Purpose

Mammalian expression of hArl8b with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF6-V5-His6

Plasmid

#67415

Purpose

Expression of hArf6 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARF1-GFP

Plasmid

#67390

Purpose

Mammalian expression of hArf1 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL2-GFP

Plasmid

#67396

Purpose

Mammalian expression of hArl2 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL4C-GFP

Plasmid

#67402

Purpose

Mammalian expression of hArl4c with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARF4-Myc

Plasmid

#67350

Purpose

Mammalian expression of hArf4 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Tags

Myc

Expression

Mammalian

Mutation

V53A, L123P and M134I mutations were unintended P…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARF5-Myc

Plasmid

#67351

Purpose

Mammalian expression of hArf5 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARF1-Myc

Plasmid

#67348

Purpose

Mammalian expression of hArf1 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pOTTC1992 - pAAV EF1a CoV2 Orf3a-2xStrep

Plasmid

#213542

Purpose

AAV viral vector packaging plasmid that expresses CoV2

Depositor

Brandon Harvey, Christopher Richie

Insert

CoV2 Orf3a-2xStrep (ORF3a Human, Severe acute respiratory syndrome coronavirus 2)

Use

AAV

Promoter

EF1a

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1993 - pAAV EF1a CoV2 protE-2xStrep

Plasmid

#213543

Purpose

AAV viral vector packaging plasmid that expresses CoV2

Depositor

Brandon Harvey, Christopher Richie

Insert

CoV2 protE-2xStrep (E Human, Severe acute respiratory syndrome coronavirus 2)

Use

AAV

Promoter

EF1a

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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hIgG1-E2-Fc

Plasmid

#194595

Purpose

Mammalian expression of E2 nanobody upstream of human IgG1; E2 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Ben Murrell

Insert

E2-Fc (S )

Expression

Mammalian

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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hIgG1-C7-Fc

Plasmid

#194596

Purpose

Mammalian expression of C7 nanobody upstream of human IgG1; C7 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Ben Murrell

Insert

C7-Fc (S )

Expression

Mammalian

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_OC43_NSP14

Plasmid

#213519

Purpose

Expresses NSP14 of hCoV OC43

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV OC43 NSP14 (ORF1ab hCoV OC43)

Tags

His-SUMO tag

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_NL63_NSP14

Plasmid

#213520

Purpose

Expresses NSP14 of hCoV NL63

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV NL63 NSP14 (ORF1ab hCoV NL63)

Tags

His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_229E_NSP14

Plasmid

#213521

Purpose

Expresses NSP14 of hCoV 229E

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV 229E NSP14

Tags

His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 ORF3a C-V5-tag

Plasmid

#179988

Purpose

Mammalian expression vector for RaTG13 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

RaTG13 ORF3a

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 M C-V5-tag

Plasmid

#179986

Purpose

Mammalian expression vector for RaTG13 M, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

RaTG13 M

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-NGFR

Plasmid

#164520

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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