Skip to main content
Addgene
Showing: 1 - 20 of 8100 results

  5. HDM_SARS2_Spike_del21_D614G

    Plasmid
    #158762
    Purpose
    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614
    Depositor
    Jesse Bloom
    Article
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Insert
    SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted amino acids 1257-1278, changed aspartic a…
    Promoter
    CMV
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158762-plasmid-map-sequence-id-309495

    Enlarge

  7. pcDNA3.3_SARS2_JN.1

    Plasmid
    #218678
    Purpose
    expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (JN.1)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L455S on the basis of BA.2.86
    Promoter
    CMV
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    218678-plasmid-map-sequence-id-437262

    Enlarge

  8. pTSara-C

    Plasmid
    #60722
    Purpose
    Expresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    Residues 180-880 of T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Only amino acids 180-880 of T7 RNAP
    Promoter
    PBAD
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60722-plasmid-map-sequence-id-97708

    Enlarge

  9. pTSara-N

    Plasmid
    #60721
    Purpose
    Expresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    Residues 1-179 of T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Only amino acids 1-179 of T7 RNAP
    Promoter
    PBAD
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60721-plasmid-map-sequence-id-97707

    Enlarge

  16. pcDNA3.3_SARS2_Mu

    Plasmid
    #177578
    Purpose
    expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus production
    Depositor
    David Nemazee
    Article
    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj
    Insert
    Spike (Mu variant) (S SARS-CoV-2)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    T95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …
    Promoter
    CMV
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177578-plasmid-map-sequence-id-348240

    Enlarge

Showing: 1 - 20 of 8100 results