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pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R

Plasmid

#228726

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-D56R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R

Plasmid

#228725

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R

Plasmid

#228729

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R

Plasmid

#228727

Purpose

Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R

Plasmid

#228728

Purpose

Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2709-CMV-Nme2Smu-ABE8e-i1-L10-D56R

Plasmid

#228730

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-D56R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1(pCMV5)

Plasmid

#12148

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 (Foxo1 Mouse)

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2007

Availability

Academic Institutions and Nonprofits only

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hIRE1a K599A

Plasmid

#20745

Depositor

Fumihiko Urano

Article

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Lentiviral; Destination vector

Expression

Mammalian

Mutation

K599A, dominant negative kinase mutant

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pEY121

Plasmid

#223468

Purpose

sra-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

sra-6 (sra-6 Nematode)

Expression

Worm

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNat-AID*-9myc

Plasmid

#99523

Purpose

C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEM3_Euk_2NLS-Flex-Cas12a (LbCas12a)-2NLS

Plasmid

#244838

Purpose

Mammalian expression of Flex-Cas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-Cas12a

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEJS2703-CMV-iNme2Smu-ABE8e-nt

Plasmid

#228724

Purpose

Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

N-terminal ABE8e with iNme2Smu nCas9 (D16A)

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R

Plasmid

#228732

Purpose

Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

Plasmid

#228735

Purpose

Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R

Plasmid

#228731

Purpose

Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS2713-CMV-iNme2-ABE8e-i1-L10

Plasmid

#228734

Purpose

Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid iNme2 nCas9 (D16A)-i1-L10

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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hGip-R

Plasmid

#14942

Depositor

Bernard Thorens

Article

Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

Insert

Gip-R (GIPR Human)

Expression

Mammalian

Mutation

Short form of the receptor

Available Since

June 7, 2007

Availability

Academic Institutions and Nonprofits only
ND4.2-Left TALE-G1397-N-dead-mCherry

Plasmid

#179683

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR

Expression

Mammalian

Mutation

E1347A

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGF-mSox2-ONL

Plasmid

#65719

Purpose

mSox2 reporter plasmid (expresses ONL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

murine Sox2 promoter and ONL

Use

Lentiviral and Luciferase

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hif-1-sgRNA-B

Plasmid

#177785

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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