We narrowed to 4,245 results for: Pet2;

1. Kif3A-EGFP-SNAP

   Plasmid

   #229852

   Purpose

   Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

   Depositor

   William Hancock

   Insert

   Kif3A (Kif3a Mouse)
   

   Use

   Tags

   6xHis, EGFP, and SNAP-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MZB215

   Plasmid

   #221767

   Purpose

   Bacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tag

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf6 (ARF6 Human)
   

   Use

   Tags

   CPD-10xHis

   Expression

   Bacterial

   Mutation

   Q67L

   Promoter

   T7 Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MZB218

   Plasmid

   #221769

   Purpose

   Bacterial expression of human Arf4 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf4 (ARF4 Human)
   

   Use

   Tags

   CPD-10xHis

   Expression

   Bacterial

   Mutation

   Q71L

   Promoter

   T7 Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MZB219

   Plasmid

   #221770

   Purpose

   Bacterial expression of human Arf5 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf5 (ARF5 Human)
   

   Use

   Tags

   CPD-10xHis

   Expression

   Bacterial

   Mutation

   Q71L

   Promoter

   T7 Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. E547* FL USP7

   Plasmid

   #229001

   Purpose

   E547-mutant of human USP7

   Depositor

   Irina Bezsonova

   Insert

   USP7 1-546 (USP7 Human)
   

   Use

   Tags

   6xHis cleavable with thrombin

   Expression

   Bacterial

   Mutation

   E547 mutated to stop codon

   Promoter

   T7

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-28a-YTH domain of YTHDC1

   Plasmid

   #228778

   Purpose

   Protein expression plasmid for YTH domain of YTHDC1 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDC1 (YTHDC1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-Cas9-FCPF

   Plasmid

   #220132

   Purpose

   Expresses Cas9-FCPF in E.coli

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   Cas9-FCPF (cas9 S. pyogenes)
   

   Use

   Tags

   His and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAM229

   Plasmid

   #226651

   Purpose

   ybbR-p97-R155H-His6

   Depositor

   Andreas Martin

   Article

   Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

   Insert

   p97 (VCP Human)
   

   Use

   Tags

   His and ybbR

   Expression

   Bacterial

   Mutation

   R155H

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAM231

   Plasmid

   #226653

   Purpose

   ybbR-p97-T262A-His6

   Depositor

   Andreas Martin

   Article

   Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

   Insert

   p97 (VCP Human)
   

   Use

   Tags

   His and ybbR

   Expression

   Bacterial

   Mutation

   T262A

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-dCas9-FCPF

    Plasmid

    #220133

    Purpose

    Expresses dCas9-FCPF in E.coli

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    dCas9-FCPF (cas9 Synthetic, S. pyogenes)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    FCPF sequence inserted on C-terminal

    Promoter

    T7

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-NIR-FbLAG30

    Plasmid

    #220748

    Purpose

    NIR-FbLAG30 expression in bacteria.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-FbLAG30
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Bacterial

    Mutation

    For bacterial cytoplasmic expression, the pelB si…

    Promoter

    T7

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SUMO-LPQTG-pET

    Plasmid

    #221851

    Purpose

    Expresses SUMO-2 with sortase motif and His tag

    Depositor

    Artur Krężel

    Article

    Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

    Insert

    SUMO-2 (SUMO2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gln88Leu, Gln89Pro

    Promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SUMO CRYGS 16-177 Q16C

    Plasmid

    #217673

    Purpose

    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation

    Depositor

    Larry David

    Insert

    CRYGS (CRYGS Human)
    

    Use

    Tags

    6XHis and SUMO

    Expression

    Bacterial

    Mutation

    Glutamine 16 changed to Cysteine, and amino acids…

    Promoter

    T7

    Available since

    May 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEL1954

    Plasmid

    #195689

    Purpose

    Expression/purification of 6xHis-TEV-MlaD(F118A/L123A)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaD(F118A/L123A)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEL2707

    Plasmid

    #195636

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL2753

    Plasmid

    #195637

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; I241N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEL2754

    Plasmid

    #195638

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; L245N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEL2755

    Plasmid

    #195639

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; I248N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEL2756

    Plasmid

    #195640

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEL2757

    Plasmid

    #195641

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge