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pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

Plasmid

#63168

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-V5"

Tags

N-myristoylation

Expression

Insect

Promoter

GAL4-responsive UAS hsp70

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4 ∆24-mCherry

Plasmid

#80271

Purpose

mammalian expression plasmid for Plk4 with multi-phospho degron deleted

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of multi-phospho degron (amino acids 282…

Promoter

CMV

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-IP GFP-Atg14

Plasmid

#38264

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only
pET29b-pduCDE

Plasmid

#110138

Purpose

Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

Insert

pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)

Expression

Bacterial

Promoter

T7

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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CFP-PBD

Plasmid

#11394

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

p21-activated kinase 1 (PAK1 Human)

Tags

CFP

Expression

Mammalian

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
EGFP-NIK

Plasmid

#111197

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

NIK (MAP3K14 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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NET33 pEGFP-N2 (597)

Plasmid

#61989

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET33 (SCARA5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Nanog

Plasmid

#40800

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Nanog (Nanog Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
Ypet-PBD

Plasmid

#105290

Purpose

To measure Rac or Cdc42 activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Pak1 (PAK1 Human)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 65-150

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PITPbeta-GFP

Plasmid

#58274

Purpose

mammalian expression of GFP-PITP beta

Depositor

Shamshad Cockcroft

Article

Morgan et al Biochem J. 2006 Sep 15;398(3):411-21.

Insert

PITP beta (PITPNB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

Plasmid

#63167

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-cMyc"

Tags

N-myristoylation

Expression

Insect

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac1 hBRM

Plasmid

#1956

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BRM (SMARCA2 Human)

Tags

flag

Expression

Insect

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
Cdk4-DN-HA

Plasmid

#1877

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk4 (CDK4 Human)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pEBB HA cIAP1 H588A

Plasmid

#38233

Depositor

Colin Duckett

Article

Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

Insert

cIAP1 H588A (BIRC2 Human)

Tags

HA

Expression

Mammalian

Mutation

H588A

Promoter

EF1a

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pMH-Cas9-gate

Plasmid

#113742

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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NET31 mRFP-N2 (490)

Plasmid

#61984

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

Insert

NET31 (TMEM209 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pRetroSuper-GFP Smad2

Plasmid

#15722

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad2 shRNA (SMAD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-Tev-Keap1

Plasmid

#62456

Purpose

His6-halo tagged human Keap1 protein for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Bacterial

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV mLST8 myc (aka: GbL-myc)

Plasmid

#12580

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mLST8 (MLST8 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

Plasmid

#63169

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-FLAG"

Tags

N-myristoylation

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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