We narrowed to 41,119 results for: gats

1. R777-E024 Hs.CDK4-nostop

   Plasmid

   #70308

   Purpose

   Gateway ORF clone of human CDK4 [NM_000075.3] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCVL TAL(Nd154C63) T7 Age-Xho/Xba-Sal linkers

   Plasmid

   #50624

   Purpose

   pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.

   Depositor

   Andrew Scharenberg

   Type

   Empty backbone

   Use

   Mrna production

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. TALEN_C18

   Plasmid

   #47974

   Purpose

   Golden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokI

   Depositor

   Gang Bao

   Insert

   Transcription Activator Like Effector Nuclease
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Shortened C-Terminus,Codon Optimized FokI Endonuc…

   Promoter

   CMV

   Available since

   Dec. 12, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pEZYflag

   Plasmid

   #18700

   Purpose

   pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a(+)-GDH

   Plasmid

   #108954

   Purpose

   heterologous expression of GDH of B. megaterium in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Glucose dehydrogenase IV (GDH IV)
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG/HA-hBIG2

   Plasmid

   #226254

   Purpose

   Expresses N-terminally HA-tagged hBIG2 in mammalian cells

   Depositor

   Hye-Won Shin

   Insert

   BIG2 (ARFGEF2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p663-UBC-V5-miniTurbo-V5-STOP_IDG-K

   Plasmid

   #158158

   Purpose

   Gateway destination clone of miniTurbo-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-miniTurbo-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIC-HIS6-MBP-TEV-GG

   Plasmid

   #215800

   Purpose

   Cloning of target gene with N terminal His6 MBP tag and TEV Cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pUS250

   Plasmid

   #198322

   Purpose

   Cumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoprotein

   Depositor

   Nicholas Coleman

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cumate-inducible

   Available since

   April 12, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. R777-E015 Hs.BRAF

    Plasmid

    #70299

    Purpose

    Gateway ORF clone of human BRAF [NM_004333.4] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. R777-E213 Hs.RAF1

    Plasmid

    #70497

    Purpose

    Gateway ORF clone of human RAF1 [NM_002880.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNIC-HIS6-NUSA-TEV-GG

    Plasmid

    #215798

    Purpose

    Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. R777-E011 Hs.ARHGAP35

    Plasmid

    #70295

    Purpose

    Gateway ORF clone of human ARHGAP35 [NM_004491.4] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    ARHGAP35 (ARHGAP35 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGGDestSC+ATG

    Plasmid

    #49323

    Purpose

    Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET24c(+)-EFTs

    Plasmid

    #31173

    Depositor

    Linda Spremulli

    Article

    Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

    Insert

    E. coli elongation factor Ts
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pEZYegfp

    Plasmid

    #18671

    Purpose

    pEGFPC4 converted Gateway Destination vector for expressing EGFP tagged fusion proteins in mammalian cells

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. p667-UBC-GFP-V5-TurboID_IDG-K

    Plasmid

    #135241

    Purpose

    Gateway destination clone of GFP (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    GFP-V5-TurboID
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p663-UBC-TurboID-V5-dsRed_IDG-K

    Plasmid

    #135261

    Purpose

    Gateway destination clone of dsRed (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-dsRed
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. R777-E063 Hs.ETS1

    Plasmid

    #70347

    Purpose

    Gateway ORF clone of human ETS1 [NM_005238.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    ETS1 (ETS1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-CMV-Flag-GFP_IDG-K

    Plasmid

    #135275

    Purpose

    Gateway destination clone of GFP (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-GFP
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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