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XZ087 SFFV-mCherry-sensor(tight)-hIgG1-TM

Plasmid

#233428

Purpose

LIDAR reporter RNA with a membrane-bound human IgG as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-sensor(tight)-hIgG1-TM

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0043 SFFV-CCR6-ADAR2dd (FLP-IN)

Plasmid

#233438

Purpose

SFFV driven CCR6-ADAR2dd (as in XZ032)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ073 (IVT) CMV-TO-T7-LIDARsensor-hybridUTR3

Plasmid

#233420

Purpose

IVT template for LIDAR reporter mRNA; mCherry as marker, EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-LIDARsensor-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ089 (IVT) CMV-TO-T7-ARRB2-MCP-hybridUTR3

Plasmid

#233422

Purpose

IVT template for ARRB2-tdMCP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-ARRB2-MCP-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ387 (IVT) CMV-TO-T7-AVPR2-V2-ADAR2dd-hybridUTR3

Plasmid

#233423

Purpose

IVT template for AVPR2-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-AVPR2-V2-ADAR2dd-hybridUTR3

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0040 Ac5-FKBP-ADARdd

Plasmid

#233411

Purpose

AC5 promoter driven FRBP-ADAR2dd for drosophila cell expression

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

Ac5

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ038 CMV-TO-IGF1R-ADAR2(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233391

Purpose

IGF1R-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IGF1R-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ031 CMV-TO-CCR6-ADAR2dd(E488Q, T501A)

Plasmid

#233393

Purpose

CCR6-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ032 CMV-TO-CCR6-V2-ADAR2dd(E488Q, T501A)

Plasmid

#233394

Purpose

CCR6-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ110 CMV-TO-signal-B2AR-V2-ADAR2dd(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233402

Purpose

_-2 adrenergic receptor-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

B2AR-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM060 CMV-TO-AVPR2-(10aa-linker)-MCP (FLP-IN)

Plasmid

#233403

Purpose

AVPR2-V2-tdMCP with a 10 amino acid linker and extra V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR2-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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YH038 PGK-TO-FRB-tm-MCP

Plasmid

#233377

Purpose

PGK promoter driven FRB fused with MCP, with a CD28 transmembrane domain in between

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FRB-CD28TM-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

PGK

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN25-Pct5-(–)-Eddh(Gp3C)

Plasmid

#233877

Purpose

Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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MDH1_KW

Plasmid

#224145

Purpose

IPTG-inducible expression of C. elegans protein MDH-1 with a chitin tag

Depositor

Katherine Walstrom

Article

Thomas et al Biochim Biophys Acta Proteins Proteom. 2

Insert

MDH-1 (F46E10.10 Nematode)

Tags

Ala-intein-CBD

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-Bmp7

Plasmid

#201909

Purpose

in vitro transcription of RNA encoding FLAG-tagged Bmp7a

Depositor

Mary Mullins

Article

Little et al Nat Cell Biol. 2009 May;11(5):637-43. do

Insert

bmp7 (bmp7a Zebrafish)

Tags

FLAG tag

Promoter

SP6

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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LDB221

Plasmid

#185427

Purpose

Bacterial expression of Nup1 C-terminal residues 777-1076 as GST fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal residues 777-1076

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB203

Plasmid

#185424

Purpose

Bacterial expression of N-terminus of NUP1 nucleoporin as GST-Nup1-N fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 N-terminal domain only

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB205

Plasmid

#185425

Purpose

Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal domain only

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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KBB101

Plasmid

#185064

Purpose

Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after the last FXFG repeat

Depositor

Ken Belanger

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal region, truncated

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFOSGE_COF2E10

Plasmid

#70929

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Elp2 (Elp2 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only