We narrowed to 30,517 results for: ide

1. pJK280

   Plasmid

   #71136

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-89 to phenylalanine; additional…

   Promoter

   T7

   Available since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR - C3orf17 sgRNA 2

   Plasmid

   #70653

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C3orf17
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCRII-Topo Tmtc4

   Plasmid

   #45869

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   TMTC4 in situ probe (Tmtc4 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   nt 1220-1798 on BC031368

   Promoter

   Available since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pMB38-HF380deltaMTBD

   Plasmid

   #37333

   Depositor

   Takahide Kon

   Article

   Kon et al Nature. 2012 Mar 7;484(7394):345-50. doi

   Insert

   Cytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)
   

   Use

   Dictyostelium expression

   Tags

   His6 and FLAG tag

   Expression

   Mutation

   deleted amino acids 1-1387 and replaced amino aci…

   Promoter

   TRE-Pmin

   Available since

   Jan. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pVDL9.3

   Plasmid

   #168299

   Purpose

   Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

   Depositor

   Víctor de Lorenzo

   Article

   Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

   Insert

   Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFR-GFP

   Plasmid

   #32751

   Purpose

   Mammalian expression of epidermal growth factor receptor (EGFR) fused to EGFP

   Depositor

   Alexander Sorkin

   Article

   Carter et al J Biol Chem. 1998 Dec 25;273(52):35000-7

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. bdSUMO-GFP-HiBit

   Plasmid

   #170989

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS2422_Nme2.Smu-ABE8e-i1

   Plasmid

   #201520

   Purpose

   Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i1

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2.Smu-ABE8e-i1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS2424_Nme2.Smu-ABE8e-i8

   Plasmid

   #201522

   Purpose

   Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i8

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2.Smu-ABE8e-i8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS2423_Nme2.Smu-ABE8e-i7

    Plasmid

    #201521

    Purpose

    Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i7

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2.Smu-ABE8e-i7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. modRNAc0-Cas9-2A-GFP

    Plasmid

    #170181

    Purpose

    IVT template plasmid for making modified Cas9GFP RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9-2A-GFP
    

    Use

    Ivt template plasmid

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. IMS-HyPer2

    Plasmid

    #60248

    Purpose

    Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    IMS-HyPer2
    

    Use

    Tags

    Mitochondrial intermembrane space targeting signa…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-A3-HA-ubiquitin

    Plasmid

    #220145

    Purpose

    Expresses A3-Ub for E3 substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    A3-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWN_U6-TRAC-miniGag-Cas9

    Plasmid

    #228959

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWN_U6-B2M-miniGag-Cas9

    Plasmid

    #228958

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX307-VHL-3F-BirA

    Plasmid

    #220147

    Purpose

    Expresses VHL-BirA for VHL substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    von Hippel-Lindau tumor suppressor (VHL Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF-1a

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWN_U6-TRAC-miniGag

    Plasmid

    #228960

    Purpose

    miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPK3079

    Plasmid

    #78741

    Purpose

    Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    AsCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57.1-DUX4-WT

    Plasmid

    #99282

    Purpose

    All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)

    Depositor

    Stephen Tapscott

    Article

    Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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