We narrowed to 6,684 results for: pCMV mammalian

1. pCMV-AID-PcrA-UGI-T2A-mTagBFP2

   Plasmid

   #221224

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA-UGI-T2A-mTagBFP2
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

   Plasmid

   #208964

   Purpose

   Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV Flag-LRRK2 K17A/K18A/R1441G

   Plasmid

   #186013

   Purpose

   Flag-LRRK2 K17A/K18A/R1441G

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   Flag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)
   

   Expression

   Mammalian

   Mutation

   See depositor's comments below.

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV10-3xFLAG-prs-hMIGA2 (170-593)

   Plasmid

   #192833

   Purpose

   Express codon-optimized human MIGA2 fragment 170-593 in Expi293 cells

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   3xFLAG-PreScission cleavage site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

   Plasmid

   #133796

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized AcrIIA1 (truncated C-terminal domain only)
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   C-terminal domain only

   Promoter

   CMV and T7

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-pLenti-ACTA2-R179 (WT) (LLH661)

   Plasmid

   #242682

   Purpose

   Plasmid for expression of ACTA2-R179 (wild-type) cDNA from a lentiviral cassette

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pLenti-ACTA2-R179_cDNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

   Plasmid

   #208379

   Purpose

   Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

   Depositor

   Daniel Hawiger

   Article

   Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   human CMV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

   Plasmid

   #208976

   Purpose

   Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   nSpCas9(H840)-BPNLS-P2A-GFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

   Plasmid

   #210504

   Purpose

   expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
   

   Expression

   Mammalian

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid

    #208969

    Purpose

    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV CHMP2B-L4D,F5D-M85E-3XHA

    Plasmid

    #242416

    Purpose

    Expression of a CHMP2B membrane binding mutant (L4D F5D) with a M85E mutation and a 3xHA tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    L4D F5D M85E

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV CHMP2B-L4D,F5D-V82E-3XHA

    Plasmid

    #242393

    Purpose

    Expression of a CHMP2B membrane binding mutant (L4D F5D) with a V82E mutation and a 3xHA tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    L4D F5D V82E

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV CHMP2B-L4D,F5D-M92E-3XHA

    Plasmid

    #242417

    Purpose

    Expression of a CHMP2B membrane binding mutant (L4D F5D) with a M92E mutation and a 3xHA tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    L4D F5D M92E

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

    Plasmid

    #208966

    Purpose

    Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

    Promoter

    CMV and T7

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

    Plasmid

    #208974

    Purpose

    Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

    Plasmid

    #208971

    Purpose

    Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-3XFLAG-renilla-luciferase-beta-globin-control

    Plasmid

    #184393

    Purpose

    Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-SpCas9-VRQR(+R221K)-P2A-EGFP (LRF95)

    Plasmid

    #242666

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + R221K mutations in SpCas9(R221K/D1135V/G12…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-SpCas9-VRQR(+N394K)-P2A-EGFP (KAC956)

    Plasmid

    #242670

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N394K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VRQR(N394K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + N394K mutations in SpCas9(N394K/D1135V/G12…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-SpCas9-VRQR(+D269A)-P2A-EGFP (LRF64)

    Plasmid

    #242668

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D269A)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VRQR(D269A)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + D269A mutations in SpCas9(D269A/D1135V/G12…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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