We narrowed to 152,943 results for: des

1. pcDNA 3.1 SIRT1-FL

   Plasmid

   #105670

   Purpose

   Mammalian expression construct of mouse SIRT1 full length isoform

   Depositor

   Ullas Kolthur-Seetharam

   Article

   Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Synonymous base changes not affecting the protein…

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA5-LAP-MPS1-WT

   Plasmid

   #114044

   Purpose

   Expression of exogenous LAP tagged MPS1 WT

   Depositor

   Geert Kops

   Article

   Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

   Insert

   MPS1-WT (TTK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT MPS1 sequence

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCWXPGR-pTF-dnTCF4

   Plasmid

   #114277

   Purpose

   TET-inducible expression of a dominant negative form of the TCF4 transcription factor

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   deletion of AA 1 to 31

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.CAG.SF-iGluSnFR.A184V

   Plasmid

   #106199

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: A184V

   Promoter

   CAG

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-HNF1A-WT-Flag-UTR

   Plasmid

   #183237

   Purpose

   Expression of FLAG tagged HNF1A

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   HNF1 homeobox A (HNF1A Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lentiGuide-Crimson

   Plasmid

   #70683

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

   Depositor

   Daniel Bauer

   Article

   Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

   Inserts

   S. pyogenes sgRNA cassette
   Crimson Reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-a and hU6

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV-EF1aGFP2AKAT7(E508Q)-W

   Plasmid

   #159293

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 E508Q
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

   Promoter

   human EF1a promoter

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.CAG.SF-iGluSnFR.S72A

   Plasmid

   #106200

   Purpose

   Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: S72A

   Promoter

   CAG

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-Trim46

   Plasmid

   #176401

   Purpose

   TRIM46 tagged with mCherry

   Depositor

   Casper Hoogenraad, Thomas Schwarz

   Article

   van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

   Insert

   trim46 (Trim46 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LIMD1_pLX307

    Plasmid

    #98349

    Purpose

    Lentiviral expression of LIMD1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    LIMD1 (LIMD1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MigR1-AE

    Plasmid

    #12431

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

    Insert

    AML1-ETO fusion (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid

    #129027

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CDK9_pLX307

    Plasmid

    #98324

    Purpose

    Lentiviral expression of CDK9

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    CDK9 (CDK9 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MTCH2_pLX307

    Plasmid

    #98354

    Purpose

    Lentiviral expression of MTCH2

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    MTCH2 (MTCH2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3 1kb prom. CD274

    Plasmid

    #107002

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1-shQKI-3373

    Plasmid

    #115456

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shQKI-3373 (QKI Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LENTICRISPR-ALDOA_sgRNA1

    Plasmid

    #201590

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    ALDOA (ALDOA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DDX39B_pLX307

    Plasmid

    #98331

    Purpose

    Lentiviral expression of DDX39B

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    DDX39B (DDX39B Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S296P

    Promoter

    E1Fa

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DGKQ gRNA (BRDN0001162275)

    Plasmid

    #77377

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DGKQ

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DGKQ (DGKQ Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAP3K3 gRNA (BRDN0001148097)

    Plasmid

    #76016

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP3K3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP3K3 (MAP3K3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. BTG1

    Plasmid

    #155425

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    BTG1 (BTG1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTRIPZ Myc2-mouse TTP WT

    Plasmid

    #107000

    Purpose

    Expresses epitope-tagged wild-type murine TTP

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    tristetraprolin (Zfp36 Mouse)
    

    Use

    Tags

    Double Myc peptide epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLKO-puro-tetON-shOGDH 2497

    Plasmid

    #110942

    Purpose

    Tet-inducible shRNA targeting OGDH

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shOGDH (OGDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO-puro-tetON-shOGDH 1714

    Plasmid

    #110941

    Purpose

    Tet-inducible shRNA targeting OGDH

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shOGDH (OGDH Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO-puro-tetON-shOGDH 2956

    Plasmid

    #110943

    Purpose

    Tet-inducible shRNA targeting OGDH

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shOGDH (OGDH Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SRC gRNA (BRDN0001145402)

    Plasmid

    #75650

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SRC gRNA (BRDN0001149457)

    Plasmid

    #75652

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SRC gRNA (BRDN0001146185)

    Plasmid

    #75651

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CDK16 gRNA (BRDN0001147125)

    Plasmid

    #76903

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK16

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK16 (CDK16 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CDK16 gRNA (BRDN0001146694)

    Plasmid

    #76901

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK16

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK16 (CDK16 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CDK16 gRNA (BRDN0001147123)

    Plasmid

    #76902

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK16

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK16 (CDK16 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. lentiCRISPR - EGFP sgRNA 2

    Plasmid

    #51761

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MASTL gRNA (BRDN0001148591)

    Plasmid

    #75989

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MASTL

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MASTL (MASTL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FHL-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129628

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    FHL-cpmTq2-Calcium-lifetime-sensor
    

    Use

    Tags

    6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV,&Rhamnose

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-Neo_CAG-Cas9

    Plasmid

    #86698

    Purpose

    Cas9 expression vector for targeting to the AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCag

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CPSF4

    Plasmid

    #155477

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF4 (CPSF4 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LRPPRC gRNA (BRDN0001145987)

    Plasmid

    #77773

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LRPPRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LRPPRC (LRPPRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LRPPRC gRNA (BRDN0001147349)

    Plasmid

    #77774

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LRPPRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LRPPRC (LRPPRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MigR1-AE9a

    Plasmid

    #12433

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Nat Med. 2006 Aug . 12(8):945-9.

    Insert

    AML1-ETO9a fusion (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AML-ETO protein with ETO exon 9a.

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SKIV2L

    Plasmid

    #156014

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SKIV2L (SKIC2 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    D3E

    Promoter

    EF-1a

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge