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His6-TEV-CRBN-midi

Plasmid

#215330

Purpose

CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

Depositor

Alessio Ciulli

Article

Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

Insert

CRBN (CRBN Human)

Tags

6xHis and TEV

Expression

Bacterial

Mutation

contains amino acids 41-187 followed by a Gly-Ser…

Promoter

lacI

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)

Plasmid

#78507

Purpose

Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labeling

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Gads SH3 SH2 SH3 domain (GRAP2 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pETcon(I-LtrI)

Plasmid

#41523

Depositor

Andrew Scharenberg

Insert

I-LtrI

Tags

3x(G4S) Linker, G3S Linker, HA Tag, and Myc Tag

Expression

Yeast

Promoter

GAL1

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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pET21b-P35

Plasmid

#11808

Depositor

Guy Salvesen

Article

Riedl et al Biochemistry. 2001 Nov 6. 40(44):13274-8

Insert

P35

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pOP437

Plasmid

#85936

Purpose

Encrypted AND gate

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

Encrypted AND gate

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP458

Plasmid

#85939

Purpose

Decrypted AND gate.

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

Decrypted AND gate

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP523

Plasmid

#85942

Purpose

Encodes araC and LambdaCI. To be used with pOP549. Encrypts pOP549.

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

araC and LambdaCI

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP440

Plasmid

#85937

Purpose

Encrypted AND gate, decryption step 1.

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

Encrypted AND gate, decryption step 1

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP447

Plasmid

#85938

Purpose

Encrypted AND gate, decryption step 2.

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

Encrypted AND gate, decryption step 2

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP587

Plasmid

#85944

Purpose

pOP576 with gRNA key

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

dCas9 and gRNA

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP588

Plasmid

#85945

Purpose

pOP576 with gRNA key

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

dCas9 and gRNA

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP589

Plasmid

#85946

Purpose

pOP576 with gRNA key

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

dCas9 and gRNA

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP590

Plasmid

#85947

Purpose

pOP576 with gRNA key

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

dCas9 and gRNA

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP591

Plasmid

#85948

Purpose

pOP576 with gRNA key

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

dCas9 and gRNA

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP592

Plasmid

#85949

Purpose

pOP576 with gRNA key

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

dCas9 and gRNA

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pOP593

Plasmid

#85950

Purpose

pOP576 with gRNA key

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

dCas9 and gRNA

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP594

Plasmid

#85951

Purpose

pOP576 with gRNA key

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

dCas9 and gRNA

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP512

Plasmid

#85940

Purpose

Bi-stable switch. From Collins lab but the origin substituted for a P15A origin.

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

Bi-stable switch

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP549

Plasmid

#85941

Purpose

Bi-stable switch, similar to pOP512 but with a lambdaCI OR_1 operator in ptrc-2 promoter driving tetR.

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

Bi-stable switch

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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PaBphP 1-497

Plasmid

#36920

Depositor

Keith Moffat

Article

Yang et al Nature. 2011 Oct 16;479(7373):428-32. do

Insert

PaBphP-PCD

Tags

His

Expression

Bacterial

Mutation

Contains the photosensory core domain coding regi…

Promoter

T7

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only