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Plasmid#72397PurposeProduces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)DepositorInsertphosphoribosylaminoimidazole synthetase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK647
Plasmid#71701PurposeProduces E. coli thioredoxin reductase (EcTrxB)DepositorInsertthioredoxin reductase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK408
Plasmid#72422PurposeProduces Acetobacter aceti 1023 Est10, C-terminal His5 tagDepositorInsertferuloyl esterase
UseTagsHis5ExpressionBacterialMutationPromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK367
Plasmid#72406PurposeProduces Acetobacter aceti 1023 Est06DepositorInsertlipase
UseTagsExpressionBacterialMutationlacks Met1 and Thr2 in annotated (v1) sequencePromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK402
Plasmid#72415PurposeProduces Acetobacter aceti 1023 Est03, N-terminal His6 tagDepositorInsert4-hydroxybenzoyl-CoA thioesterase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK580
Plasmid#71694PurposeProduces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag (AaTrxAH6)DepositorInsertthioredoxin
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK532
Plasmid#72513PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK544
Plasmid#72517PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)DepositorInsertformylglycinamidine ribonucleotide synthetase, small subunit
UseTagsExpressionBacterialMutationcontains additional Ala2PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E182 Hs.PIK3R6-nostop
Plasmid#70466PurposeGateway ORF clone of human PIK3R6 [NM_001010855.3] without stop codon (for C-terminal fusions)DepositorInsertPIK3R6 (PIK3R6 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK546
Plasmid#72518PurposeProduces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)DepositorInserthypothetical protein in purSQL operon
UseTagsExpressionBacterialMutationcontains additional Ala2PromoterT7Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E181 Hs.PIK3R6
Plasmid#70465PurposeGateway ORF clone of human PIK3R6 [NM_001010855.3] with stop codon (for native or N-terminal fusions)DepositorInsertPIK3R6 (PIK3R6 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK567
Plasmid#72522PurposeProduces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)DepositorInserthypothetical protein in purSQL operon
UseTagsExpressionBacterialMutationcontains additional Ala2PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK561
Plasmid#72283PurposeProduces Acetobacter aceti 1023 thioredoxin with Cys35>Ser mutant (AaTrxA-C35S)DepositorInsertthioredoxin
UseTagsExpressionBacterialMutationchanges cysteine-35 to serinePromoterT7Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK523
Plasmid#72442PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)DepositorInserthypothetical protein
UseTagsHis6ExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK138
Plasmid#73314PurposePas2p peroxisomal membrane targeting sequenceDepositorInsertPas2p (peroxisomal membrane targeting region)
UseTagsHis6 and c-mycExpressionYeastMutationencodes residues 1-42PromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK126
Plasmid#73337PurposeEncodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein reductase domain)DepositorInsertelectron transfer flavoprotein
UseTagsHis6ExpressionBacterialMutationencodes residues 328-685PromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK323
Plasmid#73495PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetaseDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK366
Plasmid#72405PurposeProduces Acetobacter aceti 1023 Est02DepositorInsertpeptidase S9
UseTagsExpressionBacterialMutationadditional N-terminal MVKNGS sequence, alternate …PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK377
Plasmid#72411PurposeProduces Acetobacter aceti 1023 Est05DepositorInserthypothetical protein
UseTagsExpressionBacterialMutationadditional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK322
Plasmid#73494PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutaseDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationPromotern/aAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only