We narrowed to 22 results for: Kap
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TypeBlog Post...polymerase or Kapa Hifi Polymerase work very well. Some groups have successfully used Herc II or Kapa Hifi in...the published ones, so if/when you use HercII or Kapa Hifi the reaction still does NOT work, you can try...
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Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...imaging biomolecules in live cells (Sustarsic and Kapanidis 2015), which may lead to monitoring of these processes...Central PMCID: PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the ruler to the jungle:... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...addiction systems. For example, Cara Morin and James Kaper used the hok/sok system to create strains that can...PubMed PMID: 1370544. Morin, Cara E., and James B. Kaper. "Use of stabilized luciferase-expressing plasmids... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...Cy dye-nitrilotriacetic acid (NTA) conjugates (Kapanidis, Ebright, & Ebright, 2001). These Cy/NTA conjugates...Science281.5374 (1998): 269-272. PubMed PMID: 9657724. 6. Kapanidis, Achillefs N., Yon W. Ebright, and Richard H. ... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...10.1038/s41587-022-01648-w Krolak, T., Chan, K. Y., Kaplan, L., Huang, Q., Wu, J., Zheng, Q., Kozareva, V....E., Brown, A. R., Shin, N., Kim, Y., Toong, N., Kaplow, I. M., Wirthlin, M., Zhang, X., Phan, B. N., Fox... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...can be used in the OSER assay including CytERM-mKOkappa, CytERM-mTurquoise2, and CytERM-dTomato. When... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...left for his list of the bacterial species that “ESKAPE” antibiotic treatment resulting in major problems... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...Mouse IgG2a TP1129 pTP1005 (3x cysteines) Mouse Kappa TP1170 pTP1174 (1x cysteine) pTP1172 (3x cysteines... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...PMC1368960. 10. Denning, W., Das, S., Guo, S., Xu, J., Kappes, J.C., Hel, Z. “Optimization of the transductional... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus. References... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...Meiring, J. C. M., Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova, A. (2022). Opto-katanin, ... -
Evolution of Lab Techniques
TypeBlog Post...26554401. PubMed Central PMCID: PMC4727787. 9. Hunkapiller, Tom, et al. "Large-scale and automated DNA sequence... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post...10.1038/nbt.2673 Sternberg, S. H., LaFrance, B., Kaplan, M., & Doudna, J. A. (2015). Conformational control... -
Immunology Research Plasmids and Resources
TypeCollection...immunoglobulin kappa joining 1 J1 IGKJ2 immunoglobulin kappa joining 2 J2 IGKJ3 immunoglobulin kappa joining ...immunoglobulin kappa joining 4 J4 IGKJ5 immunoglobulin kappa joining 5 J5 IGKV@ immunoglobulin kappa variable...IGKDEL immunoglobulin kappa deleting element or like IGKDE IGKJ@ immunoglobulin kappa joining group IGKJ ...IGKV1-12 immunoglobulin kappa variable 1-12 IGKV112, L19 IGKV1-13 immunoglobulin kappa variable 1-13 (gene...IGKV1-16 immunoglobulin kappa variable 1-16 IGKV116, L1 IGKV1-17 immunoglobulin kappa variable 1-17 A30, IGKV117...IGKV1-27 immunoglobulin kappa variable 1-27 A20, IGKV127 IGKV1-33 immunoglobulin kappa variable 1-33 IGKV133...immunoglobulin kappa variable 1-37 (non-functional) IGKV137, O14 IGKV1-39 immunoglobulin kappa variable 1-... -
Trimmer Lab NeuroMab Collection
TypeCollection...177477 Anti-SPHKAP [L131/17R] SPHKAP Mouse Mouse IgG2a 177478 Anti-SPHKAP [L131/20R] SPHKAP Mouse Mouse...Mouse Mouse 190511 SPHKAP scFv [L131/17] L131/17 scFv SPHKAP Mouse Mouse 190512 SPHKAP scFv [L131/20] L131...L131/20 scFv SPHKAP Mouse Mouse 190513 SPHKAP scFv [L131/27] L131/27 scFv SPHKAP Mouse Mouse 190514 Kv1.3...Mouse IgG2a 177479 Anti-SPHKAP [L131/27R] SPHKAP Mouse Mouse IgG2a 177480 Anti-GluA2 [L21/32R] GluA2 Rat ...Neurabin-1/Ppp1r9a Mouse Mouse IgG2a 220392 SPHKAP [L131/60R] SPHKAP Mouse Mouse IgG2a 220393 PICK1 [L20/23R... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...from p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian) PDGFR-derived...Endoplasmic Reticulum Sec61 BFP Gia Voeltz 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic Reticulum KDEL...dL5 FAP; mCerulean3 Marcel Bruchez 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic Reticulum KDEL... -
Chemogenetics AAV Preps
TypeCollection...PHPeB Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine... -
mTOR Pathway
TypeCollection...3 MDM2 MDM2 proto-oncogene mSin1 Also known as MAPKAP1; Mitogen-activated protein kinase associated protein... -
Ras Pathway
TypeCollection...factor, erythroid 2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK...