Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,097 results for: cas12a

Clear keywords
Showing: 381 - 400 of 1097 results

  1. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

    Plasmid
    #114092
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114092-plasmid-map-sequence-id-230539

    Enlarge

  4. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

    Plasmid
    #114080
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114080-plasmid-map-sequence-id-230482

    Enlarge

  6. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid
    #114082
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimiā€¦
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114082-plasmid-map-sequence-id-220504

    Enlarge

  8. pET-28b-T7-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1916)

    Plasmid
    #114076
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R and K607R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114076-plasmid-map-sequence-id-220531

    Enlarge

  12. pN251

    Plasmid
    #188471
    Purpose
    Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)
    Depositor
    Le Cong
    Article
    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2
    Insert
    enCas12a-HF (gift from Dr. Keith Joung)
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    188471-plasmid-map-sequence-id-370973

    Enlarge

  13. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)

    Plasmid
    #114083
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.3
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimiā€¦
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114083-plasmid-map-sequence-id-220558

    Enlarge

  14. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

    Plasmid
    #114079
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114079-plasmid-map-sequence-id-231032

    Enlarge

  15. pSLQ10878

    Plasmid
    #183964
    Purpose
    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183964-plasmid-map-sequence-id-363120

    Enlarge

  16. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available Since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

    Enlarge

  19. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

    Plasmid
    #114074
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114074-plasmid-map-sequence-id-220510

    Enlarge

Showing: 381 - 400 of 1097 results