Addgene

We narrowed to 789 results for: golden gate assembly

Showing: 381 - 400 of 789 results

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(B) BLE marker(R) (SBE262)

Plasmid

#195012

Purpose

bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+BLE+tGPD

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(R)pGPD1(D)_P1(SBE109)

Plasmid

#194993

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pGPD (D)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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(F)pGPD(G)_P2 (SBE110)

Plasmid

#194992

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pGPD (G)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(I) pADH2 (J) P3 (SBE114)

Plasmid

#187629

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pADH2 (J)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(F) pADH2 (G)_P2 (SBE113)

Plasmid

#187628

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pADH2 (G)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

Plasmid

#195054

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLDP1

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

Plasmid

#195053

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pFBA

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)

Plasmid

#195052

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pXYL for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

Plasmid

#195051

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pGPD

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)

Plasmid

#195050

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pADH2

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(F) pXYL (G) P1 (SBE216)

Plasmid

#195017

Purpose

promoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pXYL (G)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(R) pXYL (D) P1 (SBE215)

Plasmid

#195016

Purpose

promoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pXYL (D)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(R)pADH2(D)_P1(SBE214)

Plasmid

#195015

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(B) pADH2 (R)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(B) NAT marker (R) (SBE173)

Plasmid

#195014

Purpose

nourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+NAT+tNOS

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(I) pGPD (J)_P3 (SBE111)

Plasmid

#194990

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pGPD (J)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(S) insD_ku70 (Q) (SBE139)

Plasmid

#187627

Purpose

the insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/Q

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(S) insD KU70 (Q)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(P) insUP_ku70 (SBE138) (B)

Plasmid

#187626

Purpose

the insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/B

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(P) insUP KU70 (B)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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C77m

Plasmid

#120989

Purpose

MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

AmpR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C78m

Plasmid

#120990

Purpose

MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

ChlorR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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GGDestX1-Amp

Plasmid

#157652

Purpose

Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Cell-free expression (e. coli sigma-70)

Tags

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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