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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,510 results for: Pet2

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Showing: 3981 - 4000 of 7510 results

  1. MPP7

    Plasmid
    #28149
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    membrane protein, palmitoylated 7 (MPP7 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    PDZ Domain (residues 135-225)
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. NET1

    Plasmid
    #25263
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NET1:D161-D373 (NET1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids D161-D373
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. MAK3

    Plasmid
    #25499
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MAK3 (NAA50 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. USP08 (2GWF)

    Plasmid
    #25217
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    usp08.hs.0001-0142 (USP8 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    rhodanese domain, residues 181-318
    Available Since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. SULT1C2 (2AD1)

    Plasmid
    #25473
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SULT1C2 (SULT1C2 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L

    Plasmid
    #12474
    Depositor
    David Baker
    Insert
    Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    b19 only. N7K D9T D10V N14T F15Y D16E Y17I Y37L K…
    Available Since
    March 13, 2007
    Availability
    Academic Institutions and Nonprofits only

  7. ppie.001.301.W257A

    Plasmid
    #137652
    Purpose
    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.
    Depositor
    Tara Davis, Melissa Jurica
    Insert
    ppie (PPIE Human)
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    001-301-W257A
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137652-plasmid-map-sequence-id-381379

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  8. pHS29

    Plasmid
    #171205
    Purpose
    bacterial expression for HIS purification of MEG-3 with mutations in the HMG-like motif
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    meg-3 (meg-3 Nematode)
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    F705A,Y708A,Y713A, N725A
    Available Since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171205-plasmid-map-sequence-id-335784

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  9. 6WAT

    Plasmid
    #162268
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    PHF1 (PHF1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162268-plasmid-map-sequence-id-319831

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  10. 6PED

    Plasmid
    #162265
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    HEMK2 (N6AMT1 Human)
    Tags
    6xHis-Thrombin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162265-plasmid-map-sequence-id-319828

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  11. 2QNR (NEDD5)

    Plasmid
    #101273
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NEDD5 (SEPTIN2 Human)
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101273-plasmid-map-sequence-id-193507

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  12. His 3C MKK3 42-347

    Plasmid
    #87879
    Purpose
    E. coli expression of MKK3 aa 42-347
    Depositor
    Peter Mace
    Insert
    MKK3 (MAP2K3 Human)
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 42-347
    Promoter
    T7
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87879-plasmid-map-sequence-id-174922

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  13. pJK103

    Plasmid
    #73265
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73265-plasmid-map-sequence-id-134809

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  14. pJK610

    Plasmid
    #72531
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit wutg C-terminal His6 tag (AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72531-plasmid-map-sequence-id-132619

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  15. pJK540

    Plasmid
    #72446
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72446-plasmid-map-sequence-id-133865

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  16. pJK586

    Plasmid
    #72459
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Thr/Trp165>Phe mutant (AaPurE1fwf-S57T)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72459-plasmid-map-sequence-id-133898

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  17. pJK565

    Plasmid
    #72454
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Ala/Trp165>Phe mutant (AaPurE1fwf-H59A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72454-plasmid-map-sequence-id-133894

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  18. pJK578

    Plasmid
    #72457
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Ala/Trp165>Phe mutant (AaPurE1fwf-S57A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72457-plasmid-map-sequence-id-133895

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  19. pJK585

    Plasmid
    #72458
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Cys/Trp165>Phe mutant (AaPurE1fwf-S57C)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72458-plasmid-map-sequence-id-133896

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  20. pJK602

    Plasmid
    #71607
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe mutant (AaPurE1-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tyrosine-154 …
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71607-plasmid-map-sequence-id-131684

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Showing: 3981 - 4000 of 7510 results