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pAAV.hSynap.(mem).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244098

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244071

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244082

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244100

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.H348H.HaloTag

Plasmid

#244086

Purpose

Cytosolic expression of green glucose sensor (high affinity) with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Use

AAV

Tags

HaloTag

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244087

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAGFLEX.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244080

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244083

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244090

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.HaloTag

Plasmid

#244085

Purpose

Cytosolic expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

HaloTag

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC TtoS

Plasmid

#226608

Purpose

All threonines to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->GG (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 QQ pairs replaced with GG pairs

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC QtoA

Plasmid

#226609

Purpose

All glutamines to alanine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

6*His-TEV-FUS LC QtoA (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All glutamine to alanine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 12S->G

Plasmid

#226597

Purpose

12 serines to glycines

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 12S->A (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

12 serines to alanine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 12S->A

Plasmid

#226598

Purpose

12 serines to alanines

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 12S->A (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

12 serines to alanine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 12S->N

Plasmid

#226599

Purpose

12 serines to asparagines

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 12S->N (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

12 serines to asparagine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#1

Plasmid

#226603

Purpose

4 serines to alanine at residue positions 30, 44, 48, 53

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC S->A#1 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#2

Plasmid

#226604

Purpose

4 serines to alanine at residue positions 70, 84, 89, 95

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4S->A#2 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#3

Plasmid

#226605

Purpose

4 serines to alanine at residue positions 108, 117, 119, 127

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4S->A#3 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC StoG

Plasmid

#226607

Purpose

All serines to glycines

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC TtoS (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All threonine to serine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL3331 MTK234-TetO-AtoBpegRNA

Plasmid

#226668

Purpose

MTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNA

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

hU6-TetO-AtoBpegRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6-TetO

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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