We narrowed to 6,096 results for: pcDNA 3.1

1. Dimer 3 AA

   Plasmid

   #198197

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 3AA linker

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-3AA-mTurquoise2
   

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LgBit-NbALFA

   Plasmid

   #201484

   Purpose

   NanoBit detection of ALFA-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   LgBit-NbALFA
   

   Tags

   LgBit

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-RNF126 C231A

   Plasmid

   #138648

   Purpose

   Mammalian expression of full-length2 HA-tagged RNF126 with an inactivating mutation in the RING domain (C231A)

   Depositor

   Jane McGlade

   Article

   Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

   Insert

   RNF126 (RNF126 Mouse)
   

   Tags

   HA-HA

   Expression

   Mammalian

   Mutation

   C231A

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP2-NbV5

   Plasmid

   #201478

   Purpose

   BRET2 detection of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   GFP2-NbV5
   

   Tags

   GFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mVenus-IQ6 WT

   Plasmid

   #198201

   Purpose

   FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   MYO5A IQ domain (Myo5a Mouse)
   

   Tags

   mVenus

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MCP-mCherry-TIAL1

   Plasmid

   #207669

   Purpose

   To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIAL1

   Depositor

   Christine Mayr

   Article

   Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

   Insert

   TIAL1 (TIAL1 Human)
   

   Tags

   MCP-mCherry

   Expression

   Mammalian

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSSA-PMG (pXK-1649)

   Plasmid

   #208857

   Purpose

   SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.

   Depositor

   Kun Xu

   Article

   Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHDR-PMG (pXK-1650)

   Plasmid

   #208858

   Purpose

   HDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.

   Depositor

   Kun Xu

   Article

   Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MCP-mCherry-TIS11B

   Plasmid

   #207670

   Purpose

   To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11B

   Depositor

   Christine Mayr

   Article

   Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

   Insert

   TIS11B (ZFP36L1 Human)
   

   Tags

   MCP-mCherry

   Expression

   Mammalian

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ALFA-bArr2

    Plasmid

    #201497

    Purpose

    Expression of the beta-arrestin2 containing a N-terminal ALFA tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    ALFA-bArr2 (ARRB2 Human)
    

    Tags

    ALFA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAL204

    Plasmid

    #22282

    Purpose

    Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Feb. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAL149

    Plasmid

    #22275

    Purpose

    Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PhyB(1-908) (PHYB Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mCherry-Kras4BCT (prenylation sequence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. V5-bArr2

    Plasmid

    #201493

    Purpose

    Expression of the beta-arrestin2 containing a N-terminal V5 tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    V5-bArr2 (ARRB2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mVenus-IQ6 R6A

    Plasmid

    #198202

    Purpose

    FRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    MYO5A IQ domain (Myo5a Mouse)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    R6A

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. bArr2-ALFA

    Plasmid

    #201498

    Purpose

    Expression of the beta-arrestin2 containing a N-terminal ALFA tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    bArr2-ALFA (ARRB2 Human)
    

    Tags

    ALFA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. V5-bArr1

    Plasmid

    #201491

    Purpose

    Expression of the beta-arrestin1 containing a N-terminal V5 tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    V5-bArr1 (ARRB1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-CMV-jGCaMP8f F366H

    Plasmid

    #204141

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    CMV

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGP-CMV-jGCaMP8f L27A

    Plasmid

    #204140

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    CMV

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mTq2-Calmodulin

    Plasmid

    #198200

    Purpose

    FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    Calmodulin 2 (Calm2 Rat)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. FERM domain of Ezrin

    Plasmid

    #62380

    Purpose

    Expresses the FERM domain of Ezrin (N terminal portion of the protein)

    Depositor

    Guillaume Charras

    Article

    Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

    Insert

    Ezrin aa1-309 (EZR Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains FERM domain of Ezrin (N terminal portion…

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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