We narrowed to 4,436 results for: BRE

1. pLVX-CMV-Galpha-i3 G183S ONE-GO

   Plasmid

   #204338

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   Galpha-i3 G183S ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G183S mutation in Galpha-i3 (renders it RGS insen…

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pcDNA3-ALK7 K222R

   Plasmid

   #80887

   Purpose

   mammalian expression of ALK7 K222R

   Depositor

   Aristidis Moustakas

   Article

   Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

   Insert

   ALK7 (Acvr1c Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K222R

   Promoter

   CMV

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. VinTS-tCRMod-GGSGGS7

   Plasmid

   #111764

   Purpose

   Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-hSyn-Gβγ-BERKY1

   Plasmid

   #158429

   Purpose

   BRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSyn

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-YFP-GRK3ct
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. VAMP2-12aa-mCit

   Plasmid

   #53237

   Purpose

   Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Tags

   12aa-linker and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

6. VinTS-tCRMod-GGSGGS5

   Plasmid

   #111763

   Purpose

   Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGTag-P2A-QFGal4-SV40pA

   Plasmid

   #155127

   Purpose

   Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   P2A-QFGal4
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p40phox-PX (1-144)

   Plasmid

   #119123

   Purpose

   Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   p40phox-PX (1-144)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-VinculinI997A_TurboID_Venus

   Plasmid

   #200274

   Purpose

   Expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

   Depositor

   Brenton Hoffman

   Article

   Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

   Insert

   VinculinI997A_TurboID_Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   change isoleucine to alanine at 997 of vinculin

   Promoter

   T7

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-hSyn-Gαi*-BERKY3

    Plasmid

    #158428

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pLenti-hSyn-Gαq*-BERKY1

    Plasmid

    #158430

    Purpose

    BRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-GRK2(RH)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)

    Plasmid

    #117908

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p47phox-PX (1-122)

    Plasmid

    #119124

    Purpose

    Bacterial expression of human phox homology (PX) domain, p47phox-PX (1-122)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    p47phox-PX (1-122)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mPLD1-PX (71-210)

    Plasmid

    #119127

    Purpose

    Bacterial expression of human phox homology (PX) domain, mPLD1-PX (71-210)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    mPLD1-PX (71-210)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-hSyn-Gαi*-BERKY1

    Plasmid

    #158427

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

    Plasmid

    #117911

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R44A & H45Q on eDHFR

    Promoter

    T7

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6-Sp-gRNA-IDS_DF_C2c_attB_rev

    Plasmid

    #182151

    Purpose

    To insert attB-rev attachment site at IDS in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    IDS-C2c-attBrev pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

    Plasmid

    #117909

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R98A on eDHFR

    Promoter

    T7

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEmal10H – MBP-EWS_300-447 (RGG1+RRM)

    Plasmid

    #238369

    Purpose

    Expresses the RGG1-RRM (300-447) region from the RNA-binding protein EWS

    Depositor

    David Libich

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-299, 448-638

    Promoter

    T7

    Available since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEmal10H – MBP-EWS_549-638 (RGG3)

    Plasmid

    #238370

    Purpose

    Expresses the RGG3 (549-638) region from the RNA-binding protein EWS

    Depositor

    David Libich

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-548

    Promoter

    T7

    Available since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge