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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,202 results for: ENA

Showing: 10101 - 10150 of 11202 results

  2. pKM343

    Plasmid
    #71487
    Purpose
    Contains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.
    Depositor
    Kenan Murphy
    Article
    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:
    Insert
    loxP-hyg-cat-loxP
    Use
    Source of loxp-hyg-cat- loxp for pcr amplificatio…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    71487-plasmid-map-sequence-id-210536

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  5. R777-E054 Hs.EGFR-nostop

    Plasmid
    #70338
    Purpose
    Gateway ORF clone of human EGFR [NM_005228.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    EGFR (EGFR Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70338-plasmid-map-sequence-id-125787

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  6. pRDA_852

    Plasmid
    #185527
    Purpose
    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    185527-plasmid-map-sequence-id-366261

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  11. p406TDH3

    Plasmid
    #15977
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TDH3 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15977-plasmid-map-sequence-id-8462

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  16. p404TDH3

    Plasmid
    #15973
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TDH3 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15973-plasmid-map-sequence-id-8458

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  19. pcDNA3.1-MAP3K5 (R256C)

    Plasmid
    #47105
    Purpose
    Expresses mutant (R256C) human MAP3K5 in mammalian cells
    Depositor
    Yardena Samuels
    Insert
    MAP3K5 (MAP3K5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed arginine 256 to cysteine
    Promoter
    CMV
    Available since
    Oct. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. HIBADH

    Plasmid
    #38840
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HIBADH (Hibadh Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. ACADVL

    Plasmid
    #38838
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACADVL (ACADVL Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  26. ACADSB

    Plasmid
    #38839
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACADSB (Acadsb Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pSAP

    Plasmid
    #206429
    Purpose
    A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    206429-plasmid-map-sequence-id-419733

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  35. pTrig

    Plasmid
    #104576
    Purpose
    Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under Lac promoter for copy increase.
    Depositor
    Harris Wang
    Article
    Sheth et al Science. 2017 Dec 15;358(6369):1457-1461
    Insert
    PLac-repL
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104576-plasmid-map-sequence-id-201435

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  38. pCACTUS

    Plasmid
    #167559
    Purpose
    Suicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.
    Depositor
    Renato Morona
    Article
    Morona et al J Bacteriol. 1995 Feb;177(4):1059-68. do
    Type
    Empty backbone
    Use
    Suicide cloning vector, temperature sensitive.
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    167559-plasmid-map-sequence-id-332373

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  42. pAd/CMV/DAX1-IRES-nEGFP

    Plasmid
    #29752
    Depositor
    Edward McCabe
    Insert
    dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
    Use
    Tags
    EGFP, IRES, and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    No mutations; Reporter is nuclear localized EGFP;…
    Promoter
    Available since
    Oct. 21, 2011
    Availability
    Academic Institutions and Nonprofits only
    29752-plasmid-map-sequence-id-213749

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  44. GAPDHS

    Plasmid
    #39010
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GAPDHS (GAPDHS Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  45. ALDH7A1

    Plasmid
    #38866
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ALDH7A1 (Aldh7a1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  47. pRB30

    Plasmid
    #165480
    Purpose
    pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His
    Depositor
    Renato Bruni
    Article
    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2
    Type
    Empty backbone
    Use
    Tags
    TEV-FLAG-10x His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    165480-plasmid-map-sequence-id-326612

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  50. ACAD11

    Plasmid
    #39004
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACAD11 (ACAD11 Human)
    Use
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 10101 - 10150 of 11202 results