...Browse a diverse set of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based...Depositor Collections MDH CUREs Community Malate Dehydrogenase CUREs Community (MCC) Collection Blog post: ...CUREs) (Link opens in a new window) The Malate Dehydrogenase CUREs Community (MCC) (Link opens in a new window...(CUREs) centered on a single enzyme, malate dehydrogenase (MDH). The MCC is a national group of educational...Visualization to Develop Student Hypotheses in a Malate Dehydrogenase Oriented CURE. Callahan KP et al. CourseSource...attitudinal outcomes: A study of the Malate dehydrogenase CUREs Community (MCC). DeChenne-Peters et al...research in undergraduate education: The malate dehydrogenase CURE community. J Biol Chem. 2022 Sep;298(9)...

...phototoxicity, autofluorescence, and perturb biological phenomena. The technique of coupling FPs with Luciferases...et al. (2015) Fluorophore-NanoLuc BRET reporters enable sensitive in vivo optical imaging and flow cytometry...

...expression of this construct is Cre-dependent, enabling spatiotemporal control over its expression. Constitutive...differentiation by immunohistochemistry, it is not amenable to FACS.  Thus, the researchers inserted human...

...Emerging Investigators, an open-access journal that enables high school students to publish peer-reviewed scientific...

... “look-up-tables” of mutant function that could enable future clinicians to improve real time decisions...Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319...

... and why Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's work in ...:34 : Kwabena's goal to build biological science opportunities in Ghana. 46:35 - end : Kwabena's time ...Many thanks to Kwabena Duedu for taking the time to sit down and talk with us.  Kwabena Duedu, PhD is ...episode of the Addgene Podcast, we sit down with Kwabena Duedu, a researcher at the University of Health...regions and thereby accelerate their research. Kwabena came across the plasmid grant in Seeding Lab’s ...doing research at Novartis. Listen to learn how Kwabena plans to use his research experiences as well as...engineering cellulose degradation. 18:21 - 23:26 : Kwabena's plans for work in Ghana - Using local biomass ...

...development of a mouse lentiviral CRISPR library.  “This enables you to do customized genetic modification on a ...human genes with 64,751 unique guide sequences to enable both negative and positive selection screening....

...re-arrangement of DNA segments in each state of a register enables the design of primer pairs that PCR amplify in ...

... Cas14 enables high-fidelity SNP genotyping. The development of  Cas14-DETECTR will soon enable the quick...Cas14 to the mix, you get the ultimate combination enabling the detection of RNA, dsDNA, and now ssDNA. By...

...base pairs. The initial denaturation step is commonly performed at 94–98°C. Denature 30 seconds at 94°C: ...time and temperatures. Initial Denaturation for 2 minutes at 94°C. Denature for 30 seconds at 94°C. Anneal... of reagents and a basic heating and cooling (denaturing and annealing) cycle. The process became automated...withstand many heating and cooling cycles, which would denature DNA polymerases from other species. In addition...in an appropriate buffer, a series of heating (denaturing) and cooling (annealing) steps allow the Taq ...nanograms of template DNA through several cycles of denaturation, annealing, and synthesis. After the PCR is ...Chain Reaction (PCR). Basic PCR Program Initial Denaturation for 2 minutes at 94°C: This initiation step ...

...with your plasmid Resuspension buffer Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated...plasmid DNA, into solution. Add a renaturing solution to the denatured bacteria. Note: This step brings...that is compatible with the next solution. Add a denaturing solution to the resuspended bacteria. Note: This...mM EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution II... II at room temperature Solution III - Renaturing Solution (Potassium Acetate) 120 mL 5M Potassium acetate...clear and thicker as the proteins and DNA are denatured. Pro-Tip Do not vortex at this stage or the genomic...

...generating cell lines using lentiviral vectors enables long-term protein expression studies. Moreover,...resistance (e.g., puromycin, blasticidin), which enables selection of a stable cell culture after transduction...Performing antibiotic selection on transduced cells enables elimination of untransduced cells, resulting in...

... Alzheimer's Zena Werb 19138 mADAM10 deltaMP in pQCXIP cFLAG ADAM10 Flag Alzheimer's Zena Werb 19228 pBS...ERBB4 ERBB4 CMV ALS Yardena Samuels 29528 pcDNA3.1-ERBB4 (E317K) ERBB4 CMV ALS Yardena Samuels 29529 pcDNA3.1...E452K) ERBB4 CMV ALS Yardena Samuels 29530 pcDNA3.1-ERBB4 (E542K) ERBB4 CMV ALS Yardena Samuels 29531 pcDNA3.1...R544W) ERBB4 CMV ALS Yardena Samuels 29532 pcDNA3.1-ERBB4 (E563K) ERBB4 CMV ALS Yardena Samuels 29533 pcDNA3.1...dead) ERBB4 CMV ALS Yardena Samuels 29534 pcDNA3.1-ERBB4 (E836K) ERBB4 CMV ALS Yardena Samuels 29535 pcDNA3.1...(E872K) ERBB4 CMV ALS Yardena Samuels 29536 pCDF1-ERBB4 WT ERBB4 CMV ALS Yardena Samuels 29537 pCDF1-ERBB4...E317K) ERBB4 CMV ALS Yardena Samuels 29538 pCDF1-ERBB4 (E452K) ERBB4 CMV ALS Yardena Samuels 29539 pCDF1...

...multiplexed recombinases, including Cre, Flp, and VCre to enable viral expression of molecular payloads in target...recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations expressing...has been expanded to incorporate VCre in order to enable three-feature cell targeting. C) Example of viral... INTRSECT INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular...addition of these recombinase recognition sequences enables directional control of the portion of the ORF that...

...studying the function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the...digested using SapI, which will reveal sticky ends to enable the rapid ligation of annealed and phosphorylated...hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables Cre/loxP recombination and fluorescence assisted...Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H...

...min) Ramp Rate (°C/sec) # Cycles Denaturation 95 10 2 1 Denaturation 95 0.5 2 50 Annealing/Extension 60...

...interest is PCR amplified, PCR products are denatured-renatured, treated with a nuclease that cleaves DNA...Publications Get Started CRISPR is a powerful system that enables researchers to manipulate the genome like never...Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the mouse zygotes.... Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro...

...identity to be easily confirmed via sequencing, and enables the variable region and constant regions of the...given antigen. A mature B cell can clone itself to enable higher production of a single antibody, but each...blot Western blots use antibodies to identify denatured proteins separated by size on an SDS-PAGE gel....additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run...primary antibody must be able to recognize the denatured configuration of the protein. For western blots...

... on the same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic...family proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal...purification methods are more straightforward and enable direct identification of molecules associated with...editor Fusion of a Cas protein to a deaminase that enables direct base conversion in RNA or DNA without a ...2016). Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian ..., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., .... D., Gross, C. A., & Rosenberg, O. S. (2019). Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...