...Recombinant DNA Technology Techniques in chemistry enable isolation and purification of cellular components...the field of recombinant DNA technology, which enables the preparation of more managable (i.e., smaller...are relatively small (∼3000 - 6000 basepairs) to enable easy manipulation. Typically plasmids contain the...molecular cloning . The process of molecular cloning enabled scientists to break chromosomes down to study their...which contains several restriction enzyme sites, enabling easy insertion of DNA by restriction enzymes digestion... purpose of replication. The selectable marker enables selection of a population of cells that have taken...

...interest is PCR amplified, PCR products are denatured-renatured, treated with a nuclease that cleaves DNA...Publications Get Started CRISPR is a powerful system that enables researchers to manipulate the genome like never...Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the mouse zygotes.... Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro...

... on the same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic...family proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal...purification methods are more straightforward and enable direct identification of molecules associated with...editor Fusion of a Cas protein to a deaminase that enables direct base conversion in RNA or DNA without a ...2016). Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian ..., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., .... D., Gross, C. A., & Rosenberg, O. S. (2019). Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

...identity to be easily confirmed via sequencing, and enables the variable region and constant regions of the...given antigen. A mature B cell can clone itself to enable higher production of a single antibody, but each...blot Western blots use antibodies to identify denatured proteins separated by size on an SDS-PAGE gel....additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run...primary antibody must be able to recognize the denatured configuration of the protein. For western blots...

...channelrhodopsins, halorhodopsins, and other optogenetic genes enables them to be packaged in AAVs. Click here for more...released through cell lysis. Episomal stability enables long-term transgene expression in non-dividing ...would no longer be limited to small transgenes, enabling the development of further AAV applications. Return...

..., a potent and selective chemogenetic actuator enables rapid neuronal and behavioral modulations in mice...Olanzapine: A potent agonist at the hM4D(Gi) DREADD amenable to clinical translation of chemogenetics. Sci ...

...localization signal based on the LOV2 domain of Avena sativa phototropin 1 LOVETRAP reversibly sequester...local concentration near the cell surface, and enabling the ion channel to function. TULIPs Tunable light-inducible...

...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...

...Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR ...

...variant, Phoenix-gp, contains only Gag-Pol and enables additional flexibility in pseudotyping. Retroviral...