We narrowed to 13 results for: ENA

...Recombinant DNA Technology Chemistry techniques enable the isolation and purification of cellular components...recombinant DNA technology has overcome this obstacle by enabling the preparation of more manageable DNA fragments... enzymes, DNA ligases, and gel electrophoresis enabled the movement of specific fragments of DNA from ...which contains several restriction enzyme sites, enabling easy insertion of DNA by restriction enzyme digestion...are relatively small (∼3,000–6,000 base pairs) to enable easy manipulation. Typically, plasmids contain ...molecular cloning. The advent of molecular cloning enabled scientists to break chromosomes down to study their...

... on the same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic...family proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal...purification methods are more straightforward and enable direct identification of molecules associated with...editor Fusion of a Cas protein to a deaminase that enables direct base conversion in RNA or DNA without a ...2016). Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian ..., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., .... D., Gross, C. A., & Rosenberg, O. S. (2019). Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

...identity to be easily confirmed via sequencing, and enables the variable region and constant regions of the...given antigen. A mature B cell can clone itself to enable higher production of a single antibody, but each...blot Western blots use antibodies to identify denatured proteins separated by size on an SDS-PAGE gel....additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run...primary antibody must be able to recognize the denatured configuration of the protein. For western blots...

...Cloning Chloroplast Toolbox Plant Expression Scott Lenaghan 122 plasmids with chloroplast-specific genetic...Dueber) for genetic engineering of S. cerevisiae , enabling larger multi-gene constructs, multiplexed CRISPR...GoldenBraid Cloning System and Toolkit Yeast Expression Elena Caro Bernat Plasmids for generating transcriptional...

...alindromic R epeats) is a powerful system that enables researchers to manipulate the genome with relative...Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the mouse zygotes.... Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro...

...localization signal based on the LOV2 domain of Avena sativa phototropin 1 LOVETRAP reversibly sequester...local concentration near the cell surface, and enabling the ion channel to function. TULIPs Tunable light-inducible...., Stehfest, K., Fudim, R., Ramakrishnan, C., Huguenard, J. R., Hegemann, P., & Deisseroth, K. (2011)....

..., a potent and selective chemogenetic actuator enables rapid neuronal and behavioral modulations in mice...Olanzapine: A potent agonist at the hM4D(Gi) DREADD amenable to clinical translation of chemogenetics. Sci ...

...Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR ...

...strong promoter from glyceraldehyde 3-phosphage dehydrogenase; also called TDH3 or GAPDH TEF1 Constitutive...

...variant, Phoenix-gp, contains only gag-pol and enables additional flexibility in pseudotyping. Figure ...

...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...

...Lentiviral vectors can target non-dividing cells, enable stable long-term expression in a host, and exhibit...

...will no longer be limited to small transgenes, enabling the development of further AAV applications. Read...