...phototoxicity, autofluorescence, and perturb biological phenomena. The technique of coupling FPs with Luciferases...et al. (2015) Fluorophore-NanoLuc BRET reporters enable sensitive in vivo optical imaging and flow cytometry...

...expression of this construct is Cre-dependent, enabling spatiotemporal control over its expression. Constitutive...differentiation by immunohistochemistry, it is not amenable to FACS.  Thus, the researchers inserted human...

...Emerging Investigators, an open-access journal that enables high school students to publish peer-reviewed scientific...

... “look-up-tables” of mutant function that could enable future clinicians to improve real time decisions...Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319...

... and why Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's work in ...:34 : Kwabena's goal to build biological science opportunities in Ghana. 46:35 - end : Kwabena's time ...Many thanks to Kwabena Duedu for taking the time to sit down and talk with us.  Kwabena Duedu, PhD is ...episode of the Addgene Podcast, we sit down with Kwabena Duedu, a researcher at the University of Health...regions and thereby accelerate their research. Kwabena came across the plasmid grant in Seeding Lab’s ...doing research at Novartis. Listen to learn how Kwabena plans to use his research experiences as well as...engineering cellulose degradation. 18:21 - 23:26 : Kwabena's plans for work in Ghana - Using local biomass ...

...development of a mouse lentiviral CRISPR library.  “This enables you to do customized genetic modification on a ...human genes with 64,751 unique guide sequences to enable both negative and positive selection screening....

...re-arrangement of DNA segments in each state of a register enables the design of primer pairs that PCR amplify in ...

... Cas14 enables high-fidelity SNP genotyping. The development of  Cas14-DETECTR will soon enable the quick...Cas14 to the mix, you get the ultimate combination enabling the detection of RNA, dsDNA, and now ssDNA. By...

...its native state to a less folded, or denatured, one. This enables absorbing of the proteins to the microtiter...researcher and ones that happen naturally.  Sample Denaturing  First, let’s think about induced conformation...assays, such as ELISAs and Westerns, frequently denature the proteins as part of the sample prep. This ... or ELISAs may therefore often be specific to denatured versions, or even to just that particular assay...    Fig. 1: A native protein (left) is denatured, allowing the antibody to bind to a previously...you’re working with native proteins instead of denatured ones - well, don’t relax just yet. Proteins often...s configuration in your assay. Did you have a denaturing step? Do you want to capture the protein bound...

...through a matrix by charge. In a denaturing western, the protein is denatured to its primary structure and...to detect the presence or absence of a protein (Renart et al., 1979, Towbin et al., 1979, Burnett et al...Westerns are divided into two categories, native and denaturing. In a native western, the protein’s secondary... purpose of this blog, we will be focusing on denaturing westerns.  Separating a protein mix by size Sodium...content and normalize to ensure equivalent loading. Denature the samples to their primary amino acid sequence...its native state while a western blot detects denatured polypeptide chains. The protein regions available...–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark GR (1979) Transfer of proteins...

...recent blog post. Fatima Enam: Biosensors for human milk oligosaccharides Fatima Enam is currently a graduate...Fluorescent proteins have enabled scientists to pursue creative research avenues previously unavailable...Davidson and Roger Tsien played enormous roles in enabling researchers to utilize and develop these tools...Commemorative Travel Awardees: Xin Zhou and Fatima Enam. There were many excellent applicants but Xin and...fluorescent proteins and the potential for their work to enable future discoveries. Read on to learn more about...these biosensors and her future work with HMOs will enable researchers to engineer healthier microbiomes in...

... have leveraged such host-defense mechanisms to enable multiplex gene editing in a variety of cells and...knockout or regulations, it lacks proper functions to enable efficient base editing and has yet to be developed...gene editing. CRISPR-Cas9, the key component to enable efficient base editing and prime editing, is unable...process its own array but is the key component to enable efficient base editing and prime editing. How do...these constraints? The Gao lab first thought to enable MBE by using Cas12a fused base editors (dCas12a-BE...potentially treat polygenic diseases. This could also enable more convenient multiplex genome engineering of...editing with DAP array   Use DAP arrays can enable efficient multiplex gene editing, such as base ...

...Kamenyeva et al, 2015. Cellular trafficking studies enabled by fluorescent proteins The optimization of GFP...live-cell imaging (intravital imaging) studies it’s enabled are revealing the cellular behaviors that mediate...antigen presenting cells, but two-photon microscopy enabled scientists to see how chaotic and random the scanning...of fluorescent pathogens and fluorescent cells enables scientists to monitor cell interactions in diverse...proteins are excited by 750 nm to 760 nm laser light, enabling dual color imaging studies with blue or cyan proteins...photoactivable or photoconvertible proteins, as it enables tracking throughout the lifetime of the cell.     ...2009): 6379-6393. PubMed PMID: 19414791. 7. John, Beena, et al. "Dynamic imaging of CD8+ T cells and dendritic...

... not with denaturing agents like DTT or β-mercaptoethanol.  Denaturing proteins To denature your proteins...ll need to lyse and denature them. If you are intentionally running non-denatured proteins, in what is...blot, your loading buffer will not contain any denaturing agents. However, you’ll still have to lyse your... post, we are assuming that you are running a denaturing blot.  There are a number of lysing methods and... aliquot your sample(s) before adding in your denaturing and/or loading buffers, as most Bradford assays...

...focused format, where all students work on malate dehydrogenase (MDH), a citric acid cycle enzyme he studied... easily measured activity, MDH is particularly amenable to in-class labs. Provost ultimately settled on... genes required for stem and progenitor cell maintenance in planarians in Developmental Biology (Kimball...complex is required for stem and progenitor cell maintenance in planarians. Developmental Biology. 457:119... of a fusion protein: Histidine tag, malate dehydrogenase, and green fluorescent protein. Biochemistry...semester-long research approach using malate dehydrogenase as a research model. Biochemistry and Molecular...

..., which are sets of genes that, when expressed, enable cells to be visualized under ultrasound imaging...cells The first-generation mammalian ARGs (mARGs) enabled ultrasound visualization of in situ mammalian gene...scattering. The best candidate for this was the Anabaena flos-aquae gas vesicle gene cluster because of... increase expression. We were able to express Anabaena GVs in mammalian cells without the shell stiffening...nonlinear ultrasound contrast. It turned out that Anabaena mARGs did not require a booster cassette, either...regular intraperitoneal doxycycline injections to enable their tracking with ultrasound imaging (Fig. 2d...

...oncology has benefited most as cancer cells are amenable to culture in vitro. Virologists too have benefited...would seem to save time and money but cells lose provenance (this is the history of the cell line consisting...Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent identification ... Public Domain. The basics of good cell line maintenance The key is good recordkeeping, you need to have...journals strictly require evidence of cell line provenance and identity. The Nature Publishing Group recently... cell line maintenance...

...Sciences 113.13 (2016): 3509-3514. Tikhonova, Elena B., et al. "A transcription blocker isolated from... E. coli, and biosensors in E. coli: Čelešnik, Helena, et al. "Biosafety of biotechnologically important... George M. Church. "Genetically encoded sensors enable real-time observation of metabolite production....pCell-Free vectors, Gateway-compatible backbones that enable cell-free expression of proteins in both prokaryotic... a set of 88 clones in a pCellFree vector that enables protein expression in any in vitro translation ...encoded in this set range in size from 4 to 156 kDa, enabling the user to characterise the efficiency of their...

... read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening. Also, check out...the subsequent biochemical characterization, has enabled scientists to engineer a wide variety of GFPs with...Timer protein life due to a form of oxidation (dehydrogenation). MitoTimers are composed of a Timer protein...channelrhodopsins, Chronos and Chrimson, which have enabled the group to perform independent two-color optical...capability to turn RUSH and CRUSH alleles off or on enables this method to rapidly address questions of tissue-specificity...peroxidase) reporter from Alice Ting’s lab has enabled progress on both fronts – as a genetic electron...Clonetegration Classic genetic engineering methods enabling chromosomal integration of sequences in bacteria...