Skip to main content
Addgene
Showing: 1 - 20 of 43 results

  1. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...Browse a diverse set of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based...Depositor Collections MDH CUREs Community Malate Dehydrogenase CUREs Community (MCC) Collection Blog post: ...CUREs) (Link opens in a new window) The Malate Dehydrogenase CUREs Community (MCC) (Link opens in a new window...(CUREs) centered on a single enzyme, malate dehydrogenase (MDH). The MCC is a national group of educational...Visualization to Develop Student Hypotheses in a Malate Dehydrogenase Oriented CURE. Callahan KP et al. CourseSource...attitudinal outcomes: A study of the Malate dehydrogenase CUREs Community (MCC). DeChenne-Peters et al...research in undergraduate education: The malate dehydrogenase CURE community. J Biol Chem. 2022 Sep;298(9)...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... Alzheimer's Zena Werb 19138 mADAM10 deltaMP in pQCXIP cFLAG ADAM10 Flag Alzheimer's Zena Werb 19228 pBS...ERBB4 ERBB4 CMV ALS Yardena Samuels 29528 pcDNA3.1-ERBB4 (E317K) ERBB4 CMV ALS Yardena Samuels 29529 pcDNA3.1...E452K) ERBB4 CMV ALS Yardena Samuels 29530 pcDNA3.1-ERBB4 (E542K) ERBB4 CMV ALS Yardena Samuels 29531 pcDNA3.1...R544W) ERBB4 CMV ALS Yardena Samuels 29532 pcDNA3.1-ERBB4 (E563K) ERBB4 CMV ALS Yardena Samuels 29533 pcDNA3.1...dead) ERBB4 CMV ALS Yardena Samuels 29534 pcDNA3.1-ERBB4 (E836K) ERBB4 CMV ALS Yardena Samuels 29535 pcDNA3.1...(E872K) ERBB4 CMV ALS Yardena Samuels 29536 pCDF1-ERBB4 WT ERBB4 CMV ALS Yardena Samuels 29537 pCDF1-ERBB4...E317K) ERBB4 CMV ALS Yardena Samuels 29538 pCDF1-ERBB4 (E452K) ERBB4 CMV ALS Yardena Samuels 29539 pCDF1...

  3. Deisseroth INTRSECT Collection

    Type
    Collection
    ...multiplexed recombinases, including Cre, Flp, and VCre to enable viral expression of molecular payloads in target...recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations expressing...has been expanded to incorporate VCre in order to enable three-feature cell targeting. C) Example of viral... INTRSECT INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular...addition of these recombinase recognition sequences enables directional control of the portion of the ORF that...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...studying the function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the...digested using SapI, which will reveal sticky ends to enable the rapid ligation of annealed and phosphorylated...hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables Cre/loxP recombination and fluorescence assisted...Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H...

  5. Plan Your Experiment

    Type
    Collection
    ...interest is PCR amplified, PCR products are denatured-renatured, treated with a nuclease that cleaves DNA...Publications Get Started CRISPR is a powerful system that enables researchers to manipulate the genome like never...Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the mouse zygotes.... Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro...

  6. Antibody Guide

    Type
    Collection
    ...identity to be easily confirmed via sequencing, and enables the variable region and constant regions of the...given antigen. A mature B cell can clone itself to enable higher production of a single antibody, but each...blot Western blots use antibodies to identify denatured proteins separated by size on an SDS-PAGE gel....additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run...primary antibody must be able to recognize the denatured configuration of the protein. For western blots...

  7. CRISPR Guide

    Type
    Collection
    ... on the same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic...family proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal...purification methods are more straightforward and enable direct identification of molecules associated with...editor Fusion of a Cas protein to a deaminase that enables direct base conversion in RNA or DNA without a ...2016). Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian ..., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., .... D., Gross, C. A., & Rosenberg, O. S. (2019). Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

  8. Immunology Research Plasmids and Resources

    Type
    Collection
    ...MIP2B, SCYB3 CXCL5 chemokine (C-X-C motif) ligand 5 ENA-78, SCYB5 CXCL6 chemokine (C-X-C motif) ligand 6 ...the body. Cytokines Cytokines are molecules that enable cells of the immune system to communicate with ...infection with virus and, like other cytokines, enable communication between cells of the immune system...homolog 2 (Drosophila) FLJ14420, SLIL3, Slit-2 TNC tenascin C HXB, MGC167029, TN TYMP thymidine phosphorylase...Return to top Cytokines Cytokines are molecules that enable cells of the immune system to communicate with ...receptor, type 2 AT2, ATGR2, MRX88 AMBN ameloblastin (enamel matrix protein) - AMELX amelogenin (amelogenesis...

  9. Viral Vectors

    Type
    Collection
    ... to transduce non-dividing cells. These viruses enable long-term stable gene expression and are permanently...Retroviruses integrate permanently into the host genome, enabling long term transgene expression. Overview of Viral...engineered to harbor a wide array of viral genomes, enabling researchers to direct a wide array of genetic ...

  10. Zebrafish Plasmid Collection

    Type
    Collection
    ... is easy to culture large populations that are amenable to genetics, high-throughput screening and non-invasive...Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi) modulation...optogenetically controlled Cre/loxP system that enables precise temporal and spatial control of gene expression...

  11. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...With proper experimental design, biosensors can enable investigation of a signaling pathway or measurement...ligands A modular chemigenetic calcium indicator enables in vivo functional imaging with near-infrared light...genetically encoded ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain...NADPH concentration Semisynthetic sensor proteins enable metabolic assays at the point of care. Science....measurements of membrane charges A FRET sensor enables quantitative measurements of membrane charges in...

  12. Lentivirus Plasmids

    Type
    Collection
    ...viruses also integrate stably into the host genome, enabling long term transgene expression. There are some...lentivirus for ORF expression, multicistronic design enables tracking of viral transduction. Also see plasmid...for inducible knockdown, multicistronic design enables tracking of viral transduction. See article for...

  13. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TTGCTCCTTGGTTGCATCtctgtcttcattatcaCAAACATTTTCTTCATTA tetmethylcytosinedioxygenase 1 TAL3572 & TAL3573 TCAACTCGCGCATCCACAaagcgaaatgttaagaGGGTGAAGGCTTCCATGA...TCAACTCGCGCATCCACAaagcgaaatgttaagaGGGTGAAGGCTTCCATGA tetmethylcytosinedioxygenase 3 TAL3574 & TAL3575 TTATTAGGCACTTTTTTGtaaacgctgtattccaTGCACTTTCCTGTCCACA...TTATTAGGCACTTTTTTGtaaacgctgtattccaTGCACTTTCCTGTCCACA tetmethylcytosinedioxygenase 3 (previous si:ch211-220a11.1) TAL3576...

  14. CRISPR History and Development for Genome Engineering

    Type
    Collection
    .... This prokaryotic immune system is especially amenable to genome engineering, offering flexibility and...target CRISPR to a given locus makes it especially amenable to genetic screens. Pooled gRNA libraries can ... providing tips from experts in the field, and enabling access to multiple plasmids used for various platform...

  15. Brain Initiative Collection

    Type
    Collection
    ...development and application of innovative tools enabling large-scale real-time interrogation and monitoring... soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics...-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics...

  16. TALEN Engineering

    Type
    Collection
    ...Targeter (Link opens in a new window) is available to enable users to identify both dimeric TALEN and monomeric...FLASH assembly. This new version of ZiFiT Targeter enables users to search their sequences of interest for...

  17. Plant Plasmids and Resources

    Type
    Collection
    ...CRISPR resources include: A Multipurpose Toolkit enables targeted, specific modification of monocot and ... plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) Toolkit includes ...

  18. TALEN Plasmids and Kits

    Type
    Collection
    ... used for single-strand annealing assay, which enables the evaluation of TALEN activities in cultured ...-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena Torres-Padilla These plasmids were designed for ...

  19. Rett Syndrome

    Type
    Collection
    ...information, prenatal LMD microarray data, ISH image data, and anatomic reference atlases of prenatal and adult...

  20. Luciferase Plasmid Collection

    Type
    Collection
    ...Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent Imaging...signal and emission in the near-infrared range, enabling enhanced bioluminescence imaging in vivo . Luciferases...
Showing: 1 - 20 of 43 results