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c-myc promoter (4x TBE1 - mut)

Plasmid

#16598

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (4xTBE1 mut) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutant TBE1 sites

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
A337C

Plasmid

#24432

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-RIPK3 S199A

Plasmid

#78806

Purpose

To overexpress RIPK3 S199A in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Mutation

S199A

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-nTEVp

Plasmid

#179666

Purpose

Ras Binding Domain split-nTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

nTEVP

Expression

Mammalian

Mutation

K65E

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-cTEVp

Plasmid

#179667

Purpose

Ras Binding Domain split-cTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

cTEVP

Expression

Mammalian

Mutation

K65E

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRRG43-sFRP1

Plasmid

#99071

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea sFRP1 (secreted frizzled related protein 1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

sFRP1 (secreted frizzled related protein 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB3043(gRNA XI-1)

Plasmid

#73285

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-hRIP3-FL V460P

Plasmid

#61379

Purpose

Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V460Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V460 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL V458P

Plasmid

#61377

Purpose

Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V458Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
TAL2307

Plasmid

#36735

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

ERCC2-TALEN_Right (Ercc2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
MPC11 IgG2b gene

Plasmid

#127248

Purpose

Expresses intact IgG2b gene in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

IgG2b gene with exons and alt pAs (Ighg2b Mouse)

Expression

Mammalian

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 K55R

Plasmid

#78807

Purpose

To overexpress RIPK3 K55R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Mutation

K55R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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416-Met25 Kar2 printor HDEL

Plasmid

#32129

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

Printor (KLHL14 Human)

Tags

HDEL and Kar2

Expression

Yeast

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pET28-At_PRORP3-His

Plasmid

#67871

Purpose

bacterial expression of PRORP3 (At4g21900), full coding sequence + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP3 (PRORP3 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Tol2 Krt4 Src (YF)-uniRapR-cerulean

Plasmid

#45509

Depositor

Klaus Hahn, Anna Huttenlocher

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src (YF)-uniRapR-cerulean

Use

Zebrafish expression

Tags

cerulean

Mutation

Y732F

Promoter

krt4

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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CMV-CC2

Plasmid

#51221

Purpose

expression of amino acid 917-1040 of chicken DCTN1 p150Glued in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Mutation

aa917-1040

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pET22b-ecDHFR-G51PEKN

Plasmid

#118582

Purpose

Bacterial expression of E.coli DHFR G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

G51 mutated to P51; Glu-Lys-Asn residues inserted…

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRRG45-GluR1

Plasmid

#99070

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea GluR1 (Glutamate receptor 1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

GluR1 (Glutamate receptor 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMTH PKC epsilon

Plasmid

#8413

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pET-NEMOL329P

Plasmid

#11969

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOL329P (IKBKG Human)

Tags

His

Expression

Bacterial

Mutation

Leucine329 to Proline

Available Since

July 13, 2006

Availability

Academic Institutions and Nonprofits only