We narrowed to 166,040 results for: addgene

1. pNoTag-DGCR8

   Plasmid

   #164694

   Purpose

   Express DGCR8 without tag in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Insert

   DGCR8 (DGCR8 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-LAP1_2-mCherry

   Plasmid

   #159526

   Purpose

   Expresses mCherry from LAP1_2 promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

   Insert

   mCherry
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   LAP1_2

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRDM15_pCSdest

   Plasmid

   #53907

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   PRDM15 (PRDM15 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pLKO-puro-sh-mFAK B

   Plasmid

   #37018

   Depositor

   Bob Weinberg

   Article

   Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

   Insert

   sh-mFAK B (Ptk2 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 31, 2012

   Availability

   Academic Institutions and Nonprofits only

5. mc2 mCherry EF1a

   Plasmid

   #206225

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-AsCas12a_crRNA-site4 (MSP3511)

   Plasmid

   #160139

   Purpose

   T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mc2 blast

   Plasmid

   #206217

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCB11

   Plasmid

   #201111

   Purpose

   pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.

   Depositor

   Mary Dunlop

   Article

   Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:

   Inserts

   CcaR
   tetA(C)
   sfGFP
   

   Expression

   Bacterial

   Mutation

   C-to-T transition at pos. no. 1049 of tetA(C) lea…

   Promoter

   J23100 and PcpcG2-172

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-dSaCas9-KRAB-blast

   Plasmid

   #191654

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAC_N_PSA1

    Plasmid

    #111690

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    PSA1 (PSMA1 Human)
    

    Expression

    Mammalian

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PFKL_pCSdest

    Plasmid

    #53901

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    PFKL (PFKL Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pRRG757-smedwi-1

    Plasmid

    #99074

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea Smedwi-1

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

    Insert

    Smedwi-1 in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-puro-mIR-31

    Plasmid

    #26088

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    miR-31 (MIR31 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pRRG36-ChAT

    Plasmid

    #99069

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea ChAT (choline acetyltransferase)

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

    Insert

    ChAT (choline acetyltransferase) in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-Rab33bb in pCSDEST2

    Plasmid

    #140882

    Purpose

    Expression of zebrafish GFP-tagged Rab33bb in mammalian cells.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab33bb
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MRAP_pCSdest

    Plasmid

    #53833

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    MRAP (MRAP Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pEN_TT 3xFLAG Luciferase

    Plasmid

    #98391

    Purpose

    Gateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    Luciferase
    

    Use

    Entry vector for gateway cloning

    Tags

    3xFLAG

    Promoter

    TRE tight and TRE tight promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA1_Tet-inducible Luciferase reporter

    Plasmid

    #64161

    Purpose

    Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for Tet-inducible Luciferase reporter
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. CEBPalpha NGFR

    Plasmid

    #44627

    Depositor

    Ellen Rothenberg

    Article

    Del Real et al Development. 2013 Mar;140(6):1207-19. do

    Insert

    CEBPalpha (Cebpa Mouse)
    

    Use

    Retroviral

    Tags

    IRES and truncated NGFR

    Expression

    Mammalian

    Available Since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pWPXL-NP1

    Plasmid

    #72654

    Purpose

    Constitutive expression of NP1

    Depositor

    Ramon Trullas

    Article

    Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

    Insert

    NP1 (Nptx1 Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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