We narrowed to 4,413 results for: luciferase

1. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

   Plasmid

   #60224

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   KrasG12D HDR donor (Kras Mouse)
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS, No promoter, and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)GαoA-91-NLuc

   Plasmid

   #207151

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)G

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1(+)GαoB-91-NLuc

   Plasmid

   #207152

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)G

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBMN(CMV-copGFP-Luc2-Puro)

   Plasmid

   #80389

   Purpose

   To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

   Depositor

   Magnus Essand

   Article

   Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

   Insert

   copGFP-T2A-Luc2-F2A-Puromycin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. HyWnt3:Luc

   Plasmid

   #192986

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMV306hsp+LuxG13

   Plasmid

   #26161

   Depositor

   Brian Robertson, Siouxsie Wiles

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   Bacterial luciferase operon + G13 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBad-LgBiT-PhoCl1-SmBiT-MBP

   Plasmid

   #164034

   Purpose

   luciferase-based PhoCl screening construct

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   LgBiT-PhoCl1-SmBiT-MBP
   

   Use

   Tags

   His tag, T7 tag, Xpress Tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBad promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.cytGAP

   Plasmid

   #78734

   Purpose

   Expression of Ca2+ sensor GAP in the cytosol

   Depositor

   Teresa Alonso

   Article

   Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Insert

   cytGAP
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHLuc Control

   Plasmid

   #141091

   Purpose

   pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR

    Plasmid

    #160042

    Purpose

    For the auxin receiver that report on auxin.

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Inserts

    osTIR1
    BlastR
    

    Use

    Luciferase

    Tags

    mAID and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MLSE-shREN

    Plasmid

    #105583

    Purpose

    retrovirally express control shRNA with GFP marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    control shRNA targeting luciferase
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p255 pPr-CREM/Ins/CMV-STOP-luc

    Plasmid

    #8408

    Purpose

    Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM/insulators/lox/luciferase
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    Probasin, CMV

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pGL3-PTEN 3'UTR

    Plasmid

    #28104

    Depositor

    Jeffrey Rosen

    Article

    Greene et al J Cell Sci. 2010 Feb 15. 123(Pt 4):606-1

    Insert

    PTEN 3'UTR (Pten Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pCRISPaint-T2A-Nluc-3xP3-RFP

    Plasmid

    #127563

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Nluc
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-basic-Polr2a promoter_600bps

    Plasmid

    #21111

    Depositor

    Dean Tantin

    Article

    Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

    Insert

    Polr2a promoter_600bps (POLR2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pGL3-Control-miR-20-Control

    Plasmid

    #21169

    Depositor

    Joshua Mendell

    Article

    O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

    Insert

    miR-20 Sensor (MIR20A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Sensor construct insert has two miR-20 binding si…

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pTH646-2µ-RLuc

    Plasmid

    #40600

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    Renilla Luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

    Plasmid

    #162918

    Purpose

    Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    firefly luciferase/green fluorescent protein fusion with upstream IRES sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SEPLuc-IRES-Antares

    Plasmid

    #141085

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEPLuc-EMCV IRES-Antares
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pONL-N1_LAMP1

    Plasmid

    #65686

    Purpose

    Expresses LAMP1-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    LAMP1
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only