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pCAG-AmCas12b-2AeGFP

Plasmid

#121947

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AmCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pWS932

Plasmid

#123055

Purpose

Pheromone responsive domain

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

PRD [3b]

Expression

Yeast

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pWS2119

Plasmid

#123048

Purpose

PbPeptide GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

PbSte2 [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFP5V1

Plasmid

#165058

Purpose

Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1- T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRAJ31f

Plasmid

#39257

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ31 system (no terminator for the transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pNH040

Plasmid

#42152

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

GFP

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pPERPlucFL

Plasmid

#14811

Depositor

Tyler Jacks

Article

Reczek et al Mol Cancer Res. 2003 Dec. 1(14):1048-57.

Insert

PERP (Perp Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pRAJ11

Plasmid

#39244

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ11 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12

Plasmid

#39246

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12m

Plasmid

#39247

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ21

Plasmid

#39249

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ21m

Plasmid

#39250

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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psiCheck2 3xUTR

Plasmid

#40762

Purpose

Renilla luciferase reporter containing siRNA target sites

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

3 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Promoter

TK/SV40

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
Gal-Rad51 KR

Plasmid

#33232

Depositor

Hannah Klein

Article

Shah et al Mol Cell. 2010 Sep 24;39(6):862-72.

Insert

Rad51 (RAD51 Budding Yeast)

Expression

Yeast

Mutation

K191R

Promoter

Gal1

Available Since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pNH050

Plasmid

#42156

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

CFP-RT-CFP

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pRVV584

Plasmid

#108280

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG aconitase GFP

Plasmid

#66104

Purpose

expression of chicken aconitase GFP

Depositor

Felicitas Proels, Martin Scaal

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

aconitase

Use

Chicken expression

Tags

EGFP

Promoter

CAG

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only
BSP179

Plasmid

#110915

Purpose

For cloning expression cassettes by HR in yeast, Universal MosSCI compatible

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Type

Empty backbone

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRET.IL (No IRES-EGFP)

Plasmid

#1832

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
RCAS(A) Chfz-7 deltaC (CT#536)

Plasmid

#13933

Depositor

Cliff Tabin

Article

Hartmann et al Development. 2000 Jul . 127(14):3141-59.

Insert

Fz 7 (FZD7 Chicken)

Use

Retroviral; Avian expression

Mutation

C-terminal 24 aa truncated.

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only