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pKD276

Plasmid

#125069

Purpose

Expression of SO_0621 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_0621 (SO_0621 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Mutation

S286L- Please see depositor comments below

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKD250.2

Plasmid

#125077

Purpose

Expression of torR under PLtetO-1, output promoter PtorCAD129

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

torR (torR )
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSR300.3

Plasmid

#125079

Purpose

Expression of uhpA under PLtetO-1, output Promoter PuhpT99

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpA (uhpA )
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pUC 3GLA

Plasmid

#71762

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Expression

Insect

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-Craf SSAA

Plasmid

#118336

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf SSAA (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

SSAA

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHES 946

Plasmid

#112644

Purpose

blue light inducible cryptochrome based optogenetic expression system

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Budding Yeast, Synthetic, Mustard Weed)

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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C2 png genomic in Casper

Plasmid

#112971

Purpose

P element vector for transposon insertion of png genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png genomic DNA (png Fly)

Use

P insertion vector

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 M2 png-myc in Casper4

Plasmid

#112968

Purpose

P element vector for transposon insertion of Myc-tagged png into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png-myc (png Fly)

Use

P insertion vector

Tags

myc epitope

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pTC130

Plasmid

#91178

Purpose

BeYDV T-DNA vector expressing TALENs targeting tomato ANT1, selectable marker gene removed from the backbone (template for amplification of the BaeI containing fragment to create pMOD_C0000)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALENs targeting tomato ANT1

Use

TALEN

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG325-Unc-9

Plasmid

#99084

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Unc-9 (also known as h.26.12f)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Unc-9 (also known as h.26.12f) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG246-MCP

Plasmid

#99083

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea MCP (also known as h.45.8g)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

MCP (also known as h.45.8g) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG208-ActR1

Plasmid

#99077

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea ActR1 (activin receptor 1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

ActR1 (activin receptor 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG128-activin

Plasmid

#99075

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Activin (also known as activin 2)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Activin (also known as activin 2) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5HA-MAGE-112-429

Plasmid

#89131

Purpose

mammalian expression of N-terminal HA-tagged human MAGE-A11 amino acid residues 112-429

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

MAGE-A11 (MAGEA11 Human)

Tags

HA

Expression

Mammalian

Mutation

contains aa 112-429

Promoter

SV40

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-MAGE-TA360

Plasmid

#89141

Purpose

mammalian expression of full-length human MAGE-A11 amino acid residues 2-429 with TA360 phosphorylation site mutated

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

MAGE-A11 (MAGEA11 Human)

Expression

Mammalian

Mutation

T360A

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
FLAG-MAGE-112-429

Plasmid

#89128

Purpose

mammalian expression of human MAGE-A11 amino acid residues 112-429 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 112-429

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-RIPK3 K89R

Plasmid

#78808

Purpose

To overexpress -RIPK3 K89R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Mutation

K89R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 K501R

Plasmid

#78810

Purpose

To overexpress RIPK3 K501R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Mutation

K501R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 K363R

Plasmid

#78809

Purpose

To overexpress RIPK3 K363R in Mammalian Cell

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Mutation

K363R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GTF2A1L

Plasmid

#67096

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2A1L (GTF2A1L Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only