We narrowed to 5,068 results for: cre encoding plasmid

1. PB-mCherry-DMDEx23-eGFP

   Plasmid

   #211366

   Purpose

   Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

   Depositor

   Ulrich Lächelt

   Article

   Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

   Insert

   mCherry-DMDEx23-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mCherry interrupted by mdx dystrophin exon 23 bet…

   Promoter

   CAG

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMT-MjtRNA-GFP(47TAG)

   Plasmid

   #193251

   Purpose

   Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

   Depositor

   Jessica Seeliger, Peter Schultz

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Inserts

   GFP(47TAG)
   MjtRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon for Tyr47 replaced with amber stop codon TA…

   Promoter

   hsp60 and metU

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-ATF4

   Plasmid

   #192911

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of ATF4

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBiFC-PBZ-4A-VN

   Plasmid

   #110647

   Purpose

   expresses a PAR-binding (PBZ) domain fused to venus N-terminus, contains point mutations that abolish PAR binding

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Use

   Tags

   DDK and VN

   Expression

   Mammalian

   Mutation

   C379A, C385A, C421A and C427A

   Promoter

   CMV

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AP2u2-CMV-Ace2-IgG1

   Plasmid

   #226455

   Purpose

   Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

   Inserts

   Angiotensin Converting Enzyme 2
   Immunoglobulin Heavy Constant Gamma 1
   

   Use

   Tags

   6xHis Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

   Plasmid

   #187611

   Purpose

   Split luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Inserts

   WWE domain with C-terminal nLuc
   WWE domain with C-terminal cLuc
   

   Use

   Lentiviral

   Tags

   FLAG, HA, Split luciferase (cLuc), Split lucifera…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

   Plasmid

   #187612

   Purpose

   Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Inserts

   WWE domain (R163A) with C-terminal nLuc
   WWE domain with C-terminal cLuc
   

   Use

   Lentiviral

   Tags

   FLAG, HA, Split luciferase (cLuc), Split lucifera…

   Expression

   Mammalian

   Mutation

   Arg163Ala

   Promoter

   EF1A

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

   Plasmid

   #228788

   Purpose

   EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   EGFP***
   TEV protease
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

   Plasmid

   #64219

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E1B

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228787

    Purpose

    EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE30-His-CKM

    Plasmid

    #124134

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Creatine kinase (CKM Chicken)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHC_DYOLacI-R

    Plasmid

    #102922

    Purpose

    pUC-19 derivative reporter plasmid for the screening of Transcription Factors (TFs) carrying the DNA-binding domain of LacI

    Depositor

    George Church

    Article

    Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PoliIFN-γ

    Plasmid

    #107289

    Purpose

    Plasmid can be used for secretion of fish Interferon gamma

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

    Insert

    Interferon gamma
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. TrubIFN-γ

    Plasmid

    #107288

    Purpose

    Plasmid can be used for secretion of fish Interferon gamma

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

    Insert

    Interferon gamma
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. OnilIFN-γ

    Plasmid

    #107287

    Purpose

    Plasmid can be used for secretion of fish Interferon gamma

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

    Insert

    Interferon gamma
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRE:GFP

    Plasmid

    #163036

    Purpose

    FLiCRE reporter gene for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pG12-SEAP

    Plasmid

    #170271

    Purpose

    Secreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    12xUAS with TATA-box minimal promoter

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-ymScarletI-CaURA3

    Plasmid

    #118457

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection. *An updated version of this plasmid is available: #168055 pFA6a-link-ymScarletI-URA3.

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpyLigase

    Plasmid

    #51722

    Purpose

    Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

    Depositor

    Mark Howarth

    Article

    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

    Insert

    SpyLigase
    

    Use

    Tags

    His6 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-ymNeongreen-CaURA3

    Plasmid

    #125703

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection. *An updated version of this plasmid is available: #168059  pFA6a-link-ymNeongreen-URA3.

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeongreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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