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pGH312_wtGFP Q80H

Plasmid

#85409

Purpose

lentiviral expression vector for wtGFP Q80H

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP Q80H

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGH314_wtGFP S65T, Q80H

Plasmid

#85407

Purpose

lentiviral expression vector for wtGFP S65T-Q80H

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP S65T, Q80H

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSCFRSF131

Plasmid

#118402

Purpose

for structure determination

Depositor

Hideo Iwai

Article

Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

Insert

MjaTFIIB mini intein

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBHDuet50F

Plasmid

#118400

Purpose

analysis of cis-splicing by mini inteins

Depositor

Hideo Iwai

Article

Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

Insert

MvuTFIIB mini intein

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBHDuet64

Plasmid

#118401

Purpose

analysis of cis-splicing by mini inteins

Depositor

Hideo Iwai

Article

Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

Insert

MvuTFIIB mini intein

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pPtGE31_ΔPtR

Plasmid

#236260

Purpose

A version of the episome pPtGE31 with a deleted nonessential region, contains the NAT cassette

Depositor

Bogumil Karas

Article

Walker et al Nat Commun. 2026 Jan 22. doi: 10.1038/s4

Type

Empty backbone

Use

Synthetic Biology; P. tricornutum expression

Expression

Bacterial and Yeast

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPtGE31_ShBle

Plasmid

#236261

Purpose

A version of the episome pPtGE31_ΔPtR with an added ShBle cassette

Depositor

Bogumil Karas

Article

Walker et al Nat Commun. 2026 Jan 22. doi: 10.1038/s4

Type

Empty backbone

Use

Synthetic Biology; P. tricornutum expression

Expression

Bacterial and Yeast

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPtGE30

Plasmid

#236256

Purpose

Transmissible episome that contains the ShBle cassette

Depositor

Bogumil Karas

Article

Walker et al Nat Commun. 2026 Jan 22. doi: 10.1038/s4

Type

Empty backbone

Use

Synthetic Biology; P. tricornutum expression

Expression

Bacterial and Yeast

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPtGE31

Plasmid

#236257

Purpose

Transmissible episome that contains the NAT cassette

Depositor

Bogumil Karas

Article

Walker et al Nat Commun. 2026 Jan 22. doi: 10.1038/s4

Type

Empty backbone

Use

Synthetic Biology; P. tricornutum expression

Expression

Bacterial and Yeast

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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ISA179: Tagatose pathway

Plasmid

#241968

Purpose

Phagemid for the tagatose pathway. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

Tagatose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGH916

Plasmid

#230842

Purpose

Ef1a-DivA-BE3-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE3

Use

Lentiviral

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTW335g3

Plasmid

#237325

Purpose

expresses S. tuberosum RbcL RbcS-6xHis

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

S. tuberosum RbcL RbcS-6xHis

Tags

6xHis

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTW335f3

Plasmid

#237324

Purpose

expresses G. max RbcL RbcS-6xHis

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

G. max RbcL RbcS-6xHis

Tags

6xHis

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGH912

Plasmid

#230855

Purpose

Ef1a-DivA-BE3-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE3

Use

Lentiviral

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA-MS2

Plasmid

#207624

Purpose

Expresses crRNA-MS2 in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

crRNA-MS2

Use

CRISPR

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-LambdaN-MCP

Plasmid

#207626

Purpose

Expresses LambdaN-MCP fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

LambdaN-MCP

Use

Synthetic Biology

Promoter

pCMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ALFA-MCP

Plasmid

#207628

Purpose

Expresses ALFA-tagged MCP fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

ALFA-MCP

Use

Synthetic Biology

Promoter

pCMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGH907

Plasmid

#230837

Purpose

Ef1a-MS2-AIDDead-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

MS2-AIDDead

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGH518

Plasmid

#230836

Purpose

Ef1a-MS2-AID*-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

MS2-AID*

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGH896

Plasmid

#230840

Purpose

Ef1a-DivA-BE-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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