We narrowed to 25,965 results for: Vit;

1. pGEX-5X-1-LARP7

   Plasmid

   #113545

   Purpose

   Produces an N-terminal GST-tagged full length LARP7 protein.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   LARP7 (LARP7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIRES2-hPAC-FLAG

   Plasmid

   #129494

   Purpose

   Expresses human TMEM206 tagged at the C-terminus with FLAG

   Depositor

   Zhaozhu Qiu

   Article

   Yang et al Science. 2019 Apr 26;364(6438):395-399.

   Insert

   human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. SPHK2 gRNA (BRDN0001148465)

   Plasmid

   #77093

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3

   Plasmid

   #112008

   Purpose

   For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Insert

   axon-GCaMP6s-P2A-mRuby3
   

   Use

   AAV and Cre/Lox

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPHK1 gRNA (BRDN0001147454)

   Plasmid

   #76813

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK1 (SPHK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETDuet-1 Hepatitis C Virus NS3/NS4A

   Plasmid

   #61696

   Purpose

   inducible dual expression of NS3 protease along with its cofactor NS4A

   Depositor

   Michael Johnson

   Article

   Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

   Inserts

   NS4A
   NS3
   

   Use

   Tags

   his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. non-targeting control gRNA (BRDN0001145694)

   Plasmid

   #80191

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. S1PR1-DuET

   Plasmid

   #213374

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   S1PR1 (S1PR1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEM-Cas9HF1

   Plasmid

   #89961

   Purpose

   Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   Cas9HF1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. araBAD-BCD20-ClpP-BCD17-ClpX

    Plasmid

    #153301

    Purpose

    Expresses clpX and clpP in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Inserts

    clpX (clpX Escherichia coli)
    clpP (clpP Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV3Tag8li-hADARB2-3xFLAG

    Plasmid

    #165470

    Purpose

    Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRetroX-TRE3G_NT-GSDMD

    Plasmid

    #201170

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    aa1-276 only

    Promoter

    TRE3G

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. p5E--3mnx1

    Plasmid

    #74632

    Purpose

    Can be used to drive expression specifically in zebrafish motor neurons

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    -3mnx1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-CMV-Galpha-oA ONE-GO

    Plasmid

    #189732

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-oA ONE-GO
    GNAO (Gnao1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    I250 (silent mutation in YFP)

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. non-targeting control gRNA (BRDN0001148862)

    Plasmid

    #80263

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. WN10150

    Plasmid

    #80443

    Purpose

    Expresses dead (908A) AsCpf1

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    nuclease dead (908A) AsCpf1
    

    Use

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    nuclease dead (D908A)

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSP6-RNAP

    Plasmid

    #48143

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase SP6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    V314A (numbering based on NCBI Sequence ID: NP_85…

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 608 pSG5L HA RB del ex4

    Plasmid

    #10730

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del exon4 (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of exon4

    Promoter

    Available since

    Oct. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

19. 409 pSG5L HA RB (379-928)

    Plasmid

    #10734

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB (379-928) (RB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 379-928

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-Ef1a-fDIO-GCaMP6f

    Plasmid

    #128315

    Purpose

    Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    His Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX-CMV-Galpha-13 ONE-GO

    Plasmid

    #189740

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-13 ONE-GO
    GNA13 (GNA13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L342M (present in YFP but no functional consequen…

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CMV-YE1-BE3-FNLS-CMV-mCherry

    Plasmid

    #154005

    Purpose

    Express YE1-BE3-FNLS

    Depositor

    Hui Yang

    Article

    Zuo et al Nat Methods. 2020 May 18. pii: 10.1038/s

    Insert

    YE1-BE3-FNLS
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 498 pSG5L HA RB del663 (NAAIRS)

    Plasmid

    #10728

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del663 (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 664 to 669 were replaced by NAAIRS, a fl…

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

24. pAAV.Syn.NES-jRGECO1b.WPRE.SV40

    Plasmid

    #100857

    Purpose

    AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRGECO1b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFBD-Akt13C(S473D)

    Plasmid

    #86577

    Purpose

    Expresses cleavable human full-length Akt1(S473D) phosphomimetic

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130; S473D phos…

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-hsyn-GRAB_rDA-mut

    Plasmid

    #140558

    Purpose

    Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ABE8.20-d

    Plasmid

    #136301

    Purpose

    expresses ABE8.20-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-d
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124537

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG mEmerald-EB3

    Plasmid

    #168514

    Purpose

    Expression of human plus-TIP microtubule binding protein EB3 fused with mEmerald for microtubule +end live tracking

    Depositor

    Franck Polleux

    Article

    Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pWPXL-c-Myc

    Plasmid

    #36980

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pLentiPGK Puro DEST JNKKTRmCerulean3

    Plasmid

    #90232

    Purpose

    Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCS2-UTX-F-MT2

    Plasmid

    #40619

    Purpose

    Expression of enzyme-dead UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

    Insert

    UTX (KDM6A Human)
    

    Use

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Mutation

    changed Histidine 1146 and Glutamic Acid 1148 to …

    Promoter

    CMV

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

33. p2T-cmv-SpCas9-TadCBEd-V106W-BlastR

    Plasmid

    #193849

    Purpose

    Express TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEd-V106W
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a-6XHis-SUMO-SIRT2

    Plasmid

    #102622

    Purpose

    Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Tags

    6XHis-SUMO

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-37 and 357-389

    Promoter

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Halo-Tev-Flag-Ube2v2

    Plasmid

    #110070

    Purpose

    Express Halo-Tev-Flag-Ube2v2 (human) in mammalian cells

    Depositor

    Yimon Aye

    Article

    Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

    Insert

    Halo-Tev-Flag-Ube2v2 (UBE2V2 Human)
    

    Use

    Tags

    Halo-TEV-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

    Plasmid

    #201683

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GPR25-DuET

    Plasmid

    #213277

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR25 (GPR25 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MLF2-GFP-3xFLAG

    Plasmid

    #230986

    Purpose

    Expresses GFP tagged MLF2 in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

    Insert

    MLF2 (MLF2 Human)
    

    Use

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMIA3-Pansio

    Plasmid

    #214445

    Purpose

    Expresses eSpCas9 and gRNA targeting Pansio-1 GSH

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    eSpCas9-P2A-mRuby2-P2A-tp53dn
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGRG36-Kn-PA1-GFP

    Plasmid

    #79088

    Purpose

    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

    Depositor

    Frank Rosenzweig

    Article

    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

    Insert

    GFP
    

    Use

    Bacterial chromosome integration and expression o…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PA1

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge