We narrowed to 4,193 results for: Pet2

1. His6-EGFP-cPHx1

   Plasmid

   #183674

   Purpose

   PtdIns(3,4)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Use

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 169-329

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MEK1C218C222

   Plasmid

   #164640

   Purpose

   Bacterial expression plasmid for His6-MEK1C218C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S218C/S222C/C277S/C376S

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mature ISG15 C-term. Domain (79-165, W123A)

   Plasmid

   #110765

   Purpose

   Bacterial expression of mutant proISG15 C-term. domain

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   proISG15 C-term. domain (79-165, W123A) (ISG15 Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   W123A

   Promoter

   T7

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. DOT1L-CC3

   Plasmid

   #111754

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Article

   Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

   Insert

   DOT1L (DOT1L Human)
   

   Use

   Tags

   8xHis-Thrombin-SUMO-TEV

   Expression

   Bacterial

   Mutation

   Contains coiled coil #3 (residues 613-661)

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. DOT1L-CC1

   Plasmid

   #111756

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Article

   Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

   Insert

   DOT1L (DOT1L Human)
   

   Use

   Tags

   8xHis-Thrombin-SUMO-TEV

   Expression

   Bacterial

   Mutation

   contains coiled coil #1 (residues 506-555)

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. DOT1L-CC2

   Plasmid

   #111755

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Article

   Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

   Insert

   DOT1L (DOT1L Human)
   

   Use

   Tags

   8xHis-Thrombin-SUMO-TEV

   Expression

   Bacterial

   Mutation

   Contains coiled coil #2 (residues 560-608)

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. CCR5-SZ218a QTY

   Plasmid

   #162444

   Purpose

   Expression in E coli and perform ligand binding in solution

   Depositor

   Shuguang Zhang

   Article

   Qing et al iScience. 2020 Oct 28;23(12):101670. doi

   Insert

   C-C chemokine receptor type 5 (CCR5 Human)
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   All L, I, V, F in the transmembrane region mutate…

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. CCR5-SZ190b QTY

   Plasmid

   #162445

   Purpose

   Expression in E coli and perform ligand binding in solution

   Depositor

   Shuguang Zhang

   Article

   Qing et al iScience. 2020 Oct 28;23(12):101670. doi

   Insert

   C-C chemokine receptor type 5 (CCR5 Human)
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   All L, I, V, F in the transmembrane region mutate…

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDL52 (clone45)

   Plasmid

   #188578

   Purpose

   High-yield production of coenzyme F420 in E. coli

   Depositor

   Gerald Lackner

   Article

   Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

   Inserts

   ribA
   ribD
   yigB
   fbiC
   cofC
   cofD
   cofI
   cofE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimization, synthetic ribosome binding si…

   Promoter

   T7 variants

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 6MIW

    Plasmid

    #127291

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    6xHis-TEV (MHHHHHHSSGRENLYFQG)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. TRIP12-V2_A755-A841

    Plasmid

    #194759

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    TRIP12 (TRIP12 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. HisGB1-CITED2(216-269)+CBPTAZ1(340-439)

    Plasmid

    #173761

    Purpose

    Bacterial coexpression of human CITED2 CTAD with CBP TAZ1

    Depositor

    Peter Wright

    Article

    Berlow et al Nature. 2017 Mar 16;543(7645):447-451. d

    Inserts

    CITED2 (216-269) (CITED2 Human)
    CBP TAZ1 (340-439) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSumo24P7vB4

    Plasmid

    #113073

    Purpose

    Plasmid for highly efficient expression of engineered IL24 with binding affinity to cognate receptors

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

    Insert

    Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 Human, SUMO part (Brachypodium distachyon))
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSumo24P7

    Plasmid

    #113072

    Purpose

    Plasmid for highly efficient expression of engineered IL24 with mutated binding sites

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

    Insert

    Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 Human, SUMO part (Brachypodium distachyon))
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAJ1

    Plasmid

    #138379

    Purpose

    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ

    Depositor

    Seth Darst

    Article

    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11

    Inserts

    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…

    Promoter

    T7

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pABD1275

    Plasmid

    #68017

    Purpose

    PIF1 protein overproduction for purification from bacterial cells

    Depositor

    Bruce Downie

    Article

    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

    Insert

    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Codon Optimized for bacterial expression (GenScri…

    Promoter

    T7

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

18. ABL1-C-HiBiT

    Plasmid

    #234568

    Purpose

    Bacterially expressed ABL1-C-HiBiT for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    Abelson Kinase Domain (ABL1 Human)
    

    Use

    Tags

    HiBiT Tag-thrombin site-10xHis and T7 gene 10 lea…

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    T7

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HisGB1-CITED(216-242)+CBPTAZ1(340-439)

    Plasmid

    #189961

    Purpose

    Coexpression of CBP TAZ1 and CITED in E coli

    Depositor

    Peter Wright

    Article

    Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

    Inserts

    CITED2 (216-242) (CITED2 Human)
    CBP TAZ1 (340-439) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)

    Plasmid

    #189963

    Purpose

    Coexpression of CBP TAZ1 and CITED2 in E coli

    Depositor

    Peter Wright

    Article

    Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

    Inserts

    CITED2 (216-269) (CITED2 Human)
    CBP TAZ1 (340-439) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L243A, E245A, L246A

    Promoter

    t7

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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